Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-RanBP3 (Ser58) Antibody #9380

No. Size Price
9380S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9380 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 70 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-RanBP3 (Ser58) Antibody recognizes endogenous levels of RanBP3b protein only when phosphorylated at Ser58, and RanBP3a protein only when phosphorylated at corresponding residue Ser126.

Phospho-RanBP3 (Ser58) Antibody检测仅在Ser58位点磷酸化的内源性RanBP3b蛋白和仅在Ser126位点发生磷酸化的RanBP3a蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser58 of human RanBP3b protein. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源RanBP3b蛋白Ser58位点相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and COS-7 cells, serum-starved overnight and either left untreated or treated with hEGF #8916 (100 ng/ml), using Phospho-RanBP3 (Ser58) Antibody (upper) or RanBP3 Rabbit mAb (lower).

使用Phospho-RanBP3 (Ser58) Antibody (上图)或RanBP3 Rabbit mAb (下图),免疫印迹(Western Blot)分析HeLa和COS-7细胞中Phospho-RanBP3 (Ser58)和RanBP3蛋白水平。细胞经血清饥饿过夜,随后进行未处理或hEGF #8916 (100 ng/ml)处理。

Background

RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1). It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3). In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex. In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4). In some cases, as with β-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6). RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway. This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8).

RanBP3蛋白起初被鉴定作为RanGTP结合蛋白,其定位于细胞核并且涉及细胞核的输出过程(1)。它的功能通过结合CRM1作为CRM1细胞核输出的辅助因子,从而稳定RanGTP-CRM1-cargo的相互作用和促进细胞核中心蛋白复合物的关联(2,3)。在Ran-bound GTP缺少的情况下,RanBP3蛋白抑制CRM1复合物结合到细胞核中心复合物。除了CRM1之外,RanBP3蛋白也已经被证实结合到RanGEF-RCC1,并且对于RanGTP-CRM1-Cargo可增加RCC1蛋白的鸟嘌呤核苷酸交换活性(1,4)。在某些情况下,正如 β-catenin和Smad2/3,RanBP3结合可能一种Ran依赖而CRM1非依赖的方式去介导靶蛋白的细胞核输出(5,6)。通过PI3K/Akt或ERK/RSK通路使RanBP3蛋白在Ser58位点磷酸化。该磷酸化在细胞核输出中对RanBP3蛋白功能起着重要作用,很可能是由于RCC1蛋白活性的刺激(7,8)。

  1. Mueller, L. et al. (1998) FEBS Lett 427, 330-6.
  2. Lindsay, M.E. et al. (2001) J Cell Biol 153, 1391-402.
  3. Englmeier, L. et al. (2001) EMBO Rep 2, 926-32.
  4. Nemergut, M.E. et al. (2002) J Biol Chem 277, 17385-8.
  5. Hendriksen, J. et al. (2005) J Cell Biol 171, 785-97.
  6. Dai, F. et al. (2009) Dev Cell 16, 345-57.
  7. Yoon, S.O. et al. (2008) Mol Cell 29, 362-75.
  8. von Knethen, A. et al. (2010) J Cell Sci 123, 192-201.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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