Cell Signaling Technology

Product Pathways - Protein Stability

Phospho-BAP1 (Ser592) Antibody #9373

Cerebral protein 6   DUB   hucep-6  

No. Size Price
9373S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9373 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 95 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-BAP1(Ser592)抗体识别内源性的Ser592磷酸化BAP1蛋白。

Source / Purification

该多克隆抗体通过合成与人BAP1的Ser592邻近的氨基酸残基序列一致的肽段,免疫动物获得。 Western blot analysis of extracts from HeLa cells, untreated or UV-treated (50 mJ, 1 hour recovery), using Phospho-BAP1 (Ser592) Antibody (upper) or β-Actin Antibody #4967 (lower) as a loading control. 使用BAP1磷酸化(Ser592)抗体(上)或β-肌动蛋白抗体#4967(下)对未经处理或经紫外线处理(50兆焦耳,1小时恢复)的HeLa细胞中提取物的免疫印迹分析结果。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or UV-treated (50 mJ, 1 hour recovery), using Phospho-BAP1 (Ser592) Antibody (upper) or β-Actin Antibody #4967 (lower) as a loading control.

Background

BAP1(BRCA1相关蛋白1)最初鉴定为一个与BRCA1相偶联的并定位于核的泛素水解酶,具有抑制细胞增殖的功能。此蛋白属于UCH去泛素化酶家族成员,在N端含有UCH结构域,在C端含有BRCA1相互作用结构域和核定位信号序列(1)。在肺癌和乳腺癌标本中常发现 BAP1基因的频繁重组和缺失(1,2),在肿瘤细胞系和动物癌变模型中进行体内突变分析发现,其去泛素化活性和核定位信号对于其发挥肿瘤抑制作用比较重要。BAP1并不直接对自泛素化的BRCA1/BARD1 E3复合体发挥去泛素化功能(4),但其与BARD1相互作用并阻碍复合体的二聚化过程,从而抑制 BRCA1/BARD1 E3复合体活性(5)。除上述作用外, BAP1还与HCF-1相互作用并去泛素化,从而控制HCF-1的稳定性(6). BAP1上Ser592位点的磷酸化由 CST基于LC-MS/MS的分析平台PhosphoScan® 所发现(7)。

  1. Jensen, D.E. et al. (1998) Oncogene 16, 1097-112.
  2. Buchhagen, D.L. et al. (1994) Int J Cancer 57, 473-9.
  3. Ventii, K.H. et al. (2008) Cancer Res 68, 6953-62.
  4. Mallery, D.L. et al. (2002) EMBO J 21, 6755-62.
  5. Nishikawa, H. et al. (2009) Cancer Res 69, 111-9.
  6. Misaghi, S. et al. (2009) Mol Cell Biol 29, 2181-92.
  7. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications