Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RanBP3 Antibody #93706

No. Size Price
93706S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
93706 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 70, 90 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

RanBP3 Antibody recognizes endogenous levels of total RanBP3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn274 of human RanBP3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from U-118 MG, NIH/3T3, and Neuro-2a cells using RanBP3 Antibody.

IP

IP

Immunoprecipitation of RanBP3 protein from U-118 MG cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is RanBP3 Antibody. Western blot analysis was performed using RanBP3 Antibody.

Background

RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1). It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3). In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex. In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4). In some cases, as with β-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6). RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway. This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8).

  1. Mueller, L. et al. (1998) FEBS Lett 427, 330-6.
  2. Lindsay, M.E. et al. (2001) J Cell Biol 153, 1391-402.
  3. Englmeier, L. et al. (2001) EMBO Rep 2, 926-32.
  4. Nemergut, M.E. et al. (2002) J Biol Chem 277, 17385-8.
  5. Hendriksen, J. et al. (2005) J Cell Biol 171, 785-97.
  6. Dai, F. et al. (2009) Dev Cell 16, 345-57.
  7. Yoon, S.O. et al. (2008) Mol Cell 29, 362-75.
  8. von Knethen, A. et al. (2010) J Cell Sci 123, 192-201.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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