Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364


No. Size Price
9364S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9364 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 100 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb detects endogenous levels of Stat6 only when phosphorylated at Tyr641. This antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins. Cross-reactivity has been observed with receptor tyrosine kinases including phosphorylated PDGF receptor that can lead to inapproriate membrane staining.

Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb Stat6 抗体只能检测内源在Tyr641位点磷酸化的Stat6蛋白。抗体不与对应位点的tyrosine残基磷酸化的Stat蛋白交叉反应。现以观察到此抗体可与酪氨酸激酶类受体交叉反应,包括磷酸化的 PDGF 受体,从而引起不恰当的膜染色。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr641 of human Stat6.

此单克隆抗体是通过合成对应人源性Stat6 Tyr641周围的磷肽免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and ACHN cells, untreated or treated with IL-4 (15 min), using Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb (upper) or Stat6 Antibody #9362 (lower).Western免疫印迹。Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb (上图) 或 Stat6 抗体 #9362 (下图)研究未经处理或经过IL-4 (15 min) 处理的HeLa 和 ACHN 细胞的细胞提取液。


Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

受到Janus激酶激活后, Stat6 入核并调控细胞素诱导基因的表达。Stat6 通过在Tyr641位点的磷酸化激活,这对IL-4 和 IL-13的应答是必需的(1-4)。另外, 在B细胞系中Stat6被 IFN-α 激活并与 Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶 2A 也参与到 IL-4介导的 Stat6信号通路中 (7)。

  1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
  2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
  3. Hou, J. et al. (1994) Science 265, 1701-1706.
  4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
  5. Takeda, K. et al. (1996) Nature 380, 627-630.
  6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
  7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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