Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat6 (Tyr641) Antibody #9361

IL-4-Stat   sc-81525   signal transducer and activator of transcription  

No. Size Price
9361L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
9361S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9361T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
9361 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 110 Rabbit
IP 1:100
F 1:100
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

Phospho-Stat6 (Tyr641) Antibody detects endogenous levels of Stat6 only when phosphorylated at tyrosine 641. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins.

Phospho-Stat6 (Tyr641)抗体只能识别在tyrosine 641位点磷酸化的内源水平Stat6。抗体不与对应位点的tyrosine磷酸化的Stat蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr641 of human Stat6. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成对应人源性Stat6 Tyr641位点周围的肽段免疫动物而获得。 抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Daudi cells, treated with IL-4 (100 ng/ml) for the indicated times using Phospho-Stat6 (Tyr641) Antibody (upper) or Stat6 antibody (lower).Western免疫印迹。用Phospho-Stat6 (Tyr641) 抗体 (上图) 或 Stat6 抗体 (下图)研究经过 IL-4 (100 ng/ml) 处理一定时间的Daudi细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or IL-4 treated (green), using Phospho-Stat6 (Tyr641) Antibody compared to a nonspecific negative control antibody (red).流式细胞仪分析未经处理(蓝色)和经过 IL-4处理(绿色)的Ramos细胞。所用抗体为 Phospho-Stat6 (Tyr641) Antibody 与非特异性的阴性对照抗体(红色)。



Confocal immunofluorescent analysis of ACHN cells serum-starved (left) or IL-4-treated (right) using Phospho-Stat6(Tyr641) Antibody (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光。用Phospho-Stat6(Tyr641) 抗体 (绿色)分析血清饥饿(左图)或者IL-4处理(右图)。DRAQ5® #4084 (DNA荧光染料)(蓝色)。


Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

受到Janus激酶激活后, Stat6 入核并调控细胞素诱导基因的表达。Stat6 通过在Tyr641位点的磷酸化激活,这对IL-4 和 IL-13的应答是必需的(1-4)。另外, 在B细胞系中Stat6被 IFN-α 激活并与 Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶 2A 也参与到 IL-4介导的 Stat6信号通路中 (7)。

  1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
  2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
  3. Hou, J. et al. (1994) Science 265, 1701-1706.
  4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
  5. Takeda, K. et al. (1996) Nature 380, 627-630.
  6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
  7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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