Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat6 (Tyr641) Antibody #9361

IL-4-Stat   sc-81525   signal transducer and activator of transcription  

No. Size Price
9361L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9361S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9361T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9361 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 110 Rabbit
IP 1:100
F 1:100
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

Phospho-Stat6 (Tyr641) Antibody detects endogenous levels of Stat6 only when phosphorylated at tyrosine 641. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins.

Phospho-Stat6 (Tyr641)抗体只能识别在tyrosine 641位点磷酸化的内源水平Stat6。抗体不与对应位点的tyrosine磷酸化的Stat蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr641 of human Stat6. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成对应人源性Stat6 Tyr641位点周围的肽段免疫动物而获得。 抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Daudi cells, treated with IL-4 (100 ng/ml) for the indicated times using Phospho-Stat6 (Tyr641) Antibody (upper) or Stat6 antibody (lower).Western免疫印迹。用Phospho-Stat6 (Tyr641) 抗体 (上图) 或 Stat6 抗体 (下图)研究经过 IL-4 (100 ng/ml) 处理一定时间的Daudi细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or IL-4 treated (green), using Phospho-Stat6 (Tyr641) Antibody compared to a nonspecific negative control antibody (red).流式细胞仪分析未经处理(蓝色)和经过 IL-4处理(绿色)的Ramos细胞。所用抗体为 Phospho-Stat6 (Tyr641) Antibody 与非特异性的阴性对照抗体(红色)。



Confocal immunofluorescent analysis of ACHN cells serum-starved (left) or IL-4-treated (right) using Phospho-Stat6(Tyr641) Antibody (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光。用Phospho-Stat6(Tyr641) 抗体 (绿色)分析血清饥饿(左图)或者IL-4处理(右图)。DRAQ5® #4084 (DNA荧光染料)(蓝色)。


Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

受到Janus激酶激活后, Stat6 入核并调控细胞素诱导基因的表达。Stat6 通过在Tyr641位点的磷酸化激活,这对IL-4 和 IL-13的应答是必需的(1-4)。另外, 在B细胞系中Stat6被 IFN-α 激活并与 Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶 2A 也参与到 IL-4介导的 Stat6信号通路中 (7)。

  1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
  2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
  3. Hou, J. et al. (1994) Science 265, 1701-1706.
  4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
  5. Takeda, K. et al. (1996) Nature 380, 627-630.
  6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
  7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :