Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359

stat-5a   stat5 a   stat5 b  

No. Size Price
9359L 300 µl ( 30 western blots ) ¥9,380.00 现货查询 购买询价
9359S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
9359 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 90 Rabbit IgG
IP 1:50
IHC-P 1:600
F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,


Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine,

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.

Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb 抗体检测内源的在Tyr694 位点磷酸化的Stat5α 和 在Tyr699位点磷酸化的 Stat5β的蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr694 of Stat5a.

此单克隆抗体是通过合成对应Stat5a Tyr694周围的多肽免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 cells, untreated or treated with Gleevec, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb (top) and total Stat5 (3H7) Rabbit mAb #9358 (bottom).Western免疫印迹。用Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb (上图) and total Stat5 (3H7) Rabbit mAb #9358 (下图)抗体研究未经处理或经Gleevec处理的 K562 细胞的细胞提取液。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb.用Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb抗体对石蜡包埋的人类乳腺癌组织进行免疫组织化学染色。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells, untreated (green) or STI571-treated (blue) using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb.流式细胞仪分析未经处理(绿色)或经过STI571处理的K562细胞,所用抗体为Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right).


Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Stat5的激活是通过一系列的配体的结合而完成,包括 IL-2, GM-CSF,生长激素和泌乳刺激素。磷酸化Tyr694位点对 Stat5的活性是必须的(1,2)。此位点的磷酸化是在红细胞生成素的作用下由Src介导而完成(3)。 Stat5在某些白血病类型的细胞中有组成性的活性(4)。用IL-3处理的内皮细胞中有磷酸化的Stat5,这就表明它参与了血管再生和细胞的运动(5)。在不同细胞类型中 Stat5a 和 Stat5b 是被独立调控和激活的。例如,干扰素处理后在U-937细胞中Stat5a的活性较强但在HeLa细胞中Stat5b的活性较强(6)。

  1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
  2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
  4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
  5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
  6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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