Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat5 (Tyr694) (14H2) Mouse mAb #9356

stat-5a   stat5 a   stat5 b  

No. Size Price
9356S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9356 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 90 Mouse IgG1
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) (14H2) Mouse mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins.

Phospho-Stat5 (Tyr694) (14H2) Mouse mAb鼠单抗只能检测内源水平在Tyr694位点磷酸化的Stat5a蛋白和在Tyr699位点磷酸化的Stat5b蛋白。抗体不与对应位点的酪氨酸残基磷酸化的Stat蛋白交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr694 of mouse Stat5a.

此单克隆抗体是通过合成对应鼠源性Stat5a Tyr694 位点周围的磷肽免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 and TF-1 cells, untreated, STI571-treated or GM-CSF-treated as indicated, using Phospho-Stat5 (Tyr694) (14H2) Mouse mAb (upper) or Stat5 Antibody (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) (14H2) Mouse mAb (上图) 或者 Stat5 Antibody (下图)抗体研究未经处理和经过STI571或GM-CSF处理的K562 和 TF-1细胞的细胞提取液。

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Stat5的激活是通过一系列的配体的结合而完成,包括 IL-2, GM-CSF,生长激素和泌乳刺激素。磷酸化Tyr694位点对 Stat5的活性是必须的(1,2)。此位点的磷酸化是在红细胞生成素的作用下由Src介导而完成(3)。 Stat5在某些白血病类型的细胞中有组成性的活性(4)。用IL-3处理的内皮细胞中有磷酸化的Stat5,这就表明它参与了血管再生和细胞的运动(5)。在不同细胞类型中 Stat5a 和 Stat5b 是被独立调控和激活的。例如,干扰素处理后在U-937细胞中Stat5a的活性较强但在HeLa细胞中Stat5b的活性较强(6)。

  1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
  2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
  4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
  5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
  6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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