Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat5 Control Cell Extracts #9353

stat-5a   stat5 a   stat5 b   stat5a   stat5b  

No. Size Price
9353S 100 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9353 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated Stat5 Cell Extracts: Total cell extracts from serum-starved HeLa cells prepared without treatment serve as a negative control. Supplied in SDS Sample Buffer. Store at -20oC. 
 
 Phosphorylated Stat5 Cell Extracts: Total cell extracts from serum-starved HeLa cells prepared with IFN-alpha treatment serve as a positive control. Supplied in SDS Sample Buffer. Store at -20oC.

非磷酸化Stat5细胞裂解物: 经血清饥饿处理但未经其它处理的HeLa细胞的总提取液作为阴性对照。保存在 SDS 样品缓冲液中并于-20°C 保存。 
 
 磷酸化Stat5细胞裂解物:经血清饥饿处理和经IFN-alpha处理的HeLa细胞的总提取液作为阳性对照。保存在 SDS 样品缓冲液中并于-20°C 保存。

Applications

Western Blots: As controls, CST recommends using 10 ul of phosphorylated and nonphosphorylated Stat5 extracts.

Western Blot: 作为对照, CST 推荐用 10 ul 磷酸化的未磷酸化的Stat5 提取液。

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Stat5的激活是通过一系列的配体的结合而完成,包括 IL-2, GM-CSF,生长激素和泌乳刺激素。磷酸化Tyr694位点对 Stat5的活性是必须的(1,2)。此位点的磷酸化是在红细胞生成素的作用下由Src介导而完成(3)。 Stat5在某些白血病类型的细胞中有组成性的活性(4)。用IL-3处理的内皮细胞中有磷酸化的Stat5,这就表明它参与了血管再生和细胞的运动(5)。在不同细胞类型中 Stat5a 和 Stat5b 是被独立调控和激活的。例如,干扰素处理后在U-937细胞中Stat5a的活性较强但在HeLa细胞中Stat5b的活性较强(6)。

  1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
  2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
  4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
  5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
  6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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