Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat5 (Tyr694) Antibody #9351

signal transducer and activator of transcription   stat-5a   stat5 a   stat5 b  

No. Size Price
9351L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
9351S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9351 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 90 Rabbit
F 1:400
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Rat, Bovine,

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) Antibody detects endogenous levelsof Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins, but does cross-react with EGFR.

Phospho-Stat5 (Tyr694) Antibody检测内源水平在Tyr694位点磷酸化的Stat5a蛋白和在Tyr699位点磷酸化的Stat5b蛋白。抗体不与对应位点的酪氨酸残基磷酸化的Stat蛋白交叉反应,但是与EGFR交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr694 of mouse Stat5a. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成对应鼠源性Stat5a Tyr694 位点周围的磷肽免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from TF-1 cells, treated with GM-CSF (25 ng/ml) for the indicated times, using Phospho-Stat5 (Tyr694) Antibody (upper) or Stat5 antibody (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) Antibody (上图)或者Stat5 antibody (下图)抗体研究经过GM-CSF (25 ng/ml)处理一定时间的TF-1细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells, untreated (green) or STI571-treated (blue), using Phospho-Stat5 (Tyr694) Antibody.流式细胞仪研究未经处理(绿色)或经过STI571处理(蓝色) 的K562 细胞。所用抗体为Phospho-Stat5 (Tyr694)。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 BaF3 cells starved of IL-3 for 6 hours followed by induction with IL-3 for 45 minutes, and either 20 μl of Phospho-Stat5 (Tyr694) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse CIS Intron 1 Primers #5131, mouse SOCS-3 promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。BaF3细胞培养至4 x 106, 并经IL-3饥饿处理6小时后经IL-3诱导45Min,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用20 μl Phospho-Stat5 (Tyr694) 抗体 或2 μl Normal Rabbit IgG #2729 。用using SimpleChIP® Mouse CIS Intron 1 Primers #5131对富集的DNA做real-time PCR,所用引物为mouse SOCS-3 promoter primers和SimpleChIP® Mouse RPL30 Intron 2 Primers #7015。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。


Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Stat5的激活是通过一系列的配体的结合而完成,包括 IL-2, GM-CSF,生长激素和泌乳刺激素。磷酸化Tyr694位点对 Stat5的活性是必须的(1,2)。此位点的磷酸化是在红细胞生成素的作用下由Src介导而完成(3)。 Stat5在某些白血病类型的细胞中有组成性的活性(4)。用IL-3处理的内皮细胞中有磷酸化的Stat5,这就表明它参与了血管再生和细胞的运动(5)。在不同细胞类型中 Stat5a 和 Stat5b 是被独立调控和激活的。例如,干扰素处理后在U-937细胞中Stat5a的活性较强但在HeLa细胞中Stat5b的活性较强(6)。

  1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
  2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
  4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
  5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
  6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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