Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p90RSK (Thr573) Antibody #9346

P90-RSK   RSK-1   RSK-2   RSK-3   S6 KINASE-3  

No. Size Price
9346S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9346T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9346 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 90 Rabbit
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Xenopus, Zebrafish,

Specificity / Sensitivity

Phospho-p90RSK (Thr573) Antibody detects endogenous p90RSK protein only when phosphorylated at Thr573.

Phospho-p90RSK (Thr573) Antibody兔多抗能够检测内源性Thr573位点磷酸化的p90RSK蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr573 of human p90RSK. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源p90RSK蛋白Thr573位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TPA-treated (right), using Phospho-p90RSK (Thr573) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞,左侧是未经处理的样品,右侧是经TPA处理的样品,使用的抗体是Phospho-p90RSK (Thr573) Antibody(呈绿色)。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记,呈红色。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr573) Antibody (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower).Western blot方法检测细胞提取物:未经处理的和TPA(200 nM), 处理的HeLa细胞,使用的抗体是Phospho-p90RSK (Thr573) Antibody (上图) 和RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (下图).

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族,其特征是包含2个不同的功能性激酶结构域(1)和一个羧基端的细胞外信号调节激酶(ERKs)结合位点(2)。RSK激酶结构域内或以外的一些位点,包括Ser380, Thr359, Ser363和Thr573,对于激酶的激活非常重要(3)。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下,通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活(3)。

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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