Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p90RSK (Thr359/Ser363) Antibody #9344

P90-RSK   RSK-1   RSK-2   RSK-3   S6 KINASE-3  

No. Size Price
9344S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9344 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 90 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-p90RSK (Thr359/Ser363) Antibody detects endogenous levels of p90RSK only when phosphorylated at Thr359/Ser363. This antibody does not recognize p90RSK phosphorylated at other sites. It shows some cross-reactivity with RSK3 when phosphorylated at the homologous sites, but no cross-reactivity with phospho-RSK2 or -MSK1.

Phospho-p90RSK (Thr359/Ser363) Antibody兔多抗能够检测内源性Thr359/Ser363位点磷酸化的p90RSK蛋白水平。该抗体不能识别其他位点磷酸化的p90RSK蛋白。该抗体能与一些同源丝氨酸残基磷酸化的RSK2 、RSK3发生交叉反应,但是不能与磷酸化的RSK2、MSK1发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of rat RSK1. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与大鼠RSK1蛋白Thr359/Ser363位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and C6 cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr359/Ser363) Antibody (upper) or control RSK1/RSK2/RSK3 Rabbit mAb #9355 (lower).Western blot方法检测细胞提取物:未经处理的和TPA(200 nM)处理的HeLa 、C6 细胞,使用的抗体是Phospho-p90RSK (Thr359/Ser363) Antibody (上图)和control RSK1/RSK2/RSK3 Rabbit mAb #9355 (下图)。

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族,其特征是包含2个不同的功能性激酶结构域(1)和一个羧基端的细胞外信号调节激酶(ERKs)结合位点(2)。RSK激酶结构域内或以外的一些位点,包括Ser380, Thr359, Ser363和Thr573,对于激酶的激活非常重要(3)。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下,通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活(3)。

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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