Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3α (Ser21) (46H12) Mouse mAb #9337

glycogen synthase kinase   gsk 3   gsk-3   gsk3  

No. Size Price
9337S 100 µl ( 20 western blots ) ¥3,900.00 现货查询 购买询价
9337 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Monkey,Zebrafish, Endogenous 51 Mouse IgG2b

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-GSK-3alpha (Ser21) (46H12) Mouse mAb detects endogenous levels of GSK-3alpha only when phosphorylated at serine 21.

Phospho-GSK-3α (Ser21) (46H12) Mouse mAb鼠单抗可以检测Ser21位点发生磷酸化的内源性GSK-3α水平 。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3alpha.

单克隆抗体是采用合成的与人源GSK-3α蛋白Ser21周围序列相对应的磷酸肽免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, control and serum treated, using Phospho-GSK-3alpha (Ser21) (46H12) Mouse mAb (top), Phospho-GSK-3alpha/beta (Ser21/9) Antibody #9331 (middle) and GSK-3beta Antibody (bottom).

Western blot 分析NIH/3T3 细胞,对照组和血清处理组,所用抗体为Phospho-GSK-3α (Ser21) (46H12) Mouse mAb 兔单抗(上), Phospho-GSK-3α/β (Ser21/9) Antibody #9331 (中) 和GSK-3β Antibody (下)。

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节Cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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