Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3β (Ser9) Antibody #9336

Glycogen synthase kinase   GSK-3beta   GSK3  

No. Size Price
9336L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9336S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9336 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 46 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Zebrafish, Bovine,

Specificity / Sensitivity

Phospho-GSK-3beta (Ser9) Antibody detects endogenous levels of GSK-3β only when phosphorylated at serine 9. The antibody may cross-react weakly with the phosphorylated form of GSK-3α due to high sequence homology.

Phospho-GSK-3β (Ser9) Antibody可以检测Ser9位点发生磷酸化的内源性GSK-3β 水平,由于具有很高的同源性,此抗体可以与GSK-3α的磷酸化形式发生轻度交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of human GSK-3β. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用合成的与人源GSK-3β蛋白序列相对应的磷酸肽免疫动物生产的,抗体由protein A和肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, λ phosphatase treated or untreated, using Phospho-GSK-3bβ (Ser9) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Western blot 分析NIH/3T3 细胞, λ磷酸酶处理组和未处理,所用抗体为Phospho-GSK-3bβ (Ser9) Antibody (上) 或 GSK-3β (27C10) Rabbit mAb #9315 (下).


Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

最初人们认为糖原合酶激酶-3(GSK-3)在胰岛素的刺激下参与调节糖原合成(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3 Kinase/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21和GSK-3β的Ser9磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节cyclin D1 蛋白裂解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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