Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3α/β (Ser21/9) Antibody #9331

Glycogen synthase kinase   gsk-3β   GSK3   gsk3β  

No. Size Price
9331S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9331 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Zebrafish, Endogenous 46 GSK-3beta. 51 GSK-3alpha. Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-GSK-3alpha/beta (Ser21/9) Antibody detects endogenous levels of GSK-3 only when phosphorylated at serine 21 of GSK-3alpha or serine 9 of GSK-3beta.

Phospho-GSK-3α/β (Ser21/9) Antibody可以检测GSK-3α Ser21位点或GSK-3β Ser9位点发生磷酸化的内源性GSK-3蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of human GSK-3 alpha. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用合成的与人源GSK-3α蛋白序列相对应的磷酸肽免疫动物生产的,抗体由protein A和肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or insulin treated for the indicated times, using Phospho-GSK-3alpha/beta (Ser21/9) Antibody (upper) or GSK-3beta Antibody #9332 (lower).

Western blot 分析293细胞胰岛素非处理组和处理组,所用抗体为PhosphoGSK-3α/β (Ser21/9) Antibody (上) 或 GSK-3β Antibody #9332 (下).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using Phospho-GSK-3alpha/beta (Ser21/9) Antibody.

免疫组化分析石蜡包埋的人乳腺癌细胞,细胞核和细胞浆定位如图,所用抗体为Phospho-GSK-3α/β (Ser21/9) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right), using Phospho-GSK-3alpha/beta (Ser21/9) Antibody. Note the reduction of stain in the LY294002-treated cells.

免疫组化分析石蜡包埋的LNCaP细胞,分为未处理组(左)和 LY294002处理组(右),使用的抗体为Phospho-GSK-3alpha/beta (Ser21/9) Antibody。注意:LY294002处理组细胞的染色减少。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-GSK-3alpha/beta (Ser21/9) Antibody.

免疫组化分析石蜡包埋的人乳腺癌细胞,分为未处理组(左)和λ磷酸酶处理组(右),使用的抗体为Phospho-GSK-3alpha/beta (Ser21/9) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-GSK-3alpha/beta (Ser21/9) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

免疫组化分析石蜡包埋的人肺癌细胞,在对照肽(左)和抗原特异性肽(右)存在条件下使用Phospho-GSK-3α/β (Ser21/9) Antibody。


Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
  6. Devi, L. and Ohno, M. (2015) Transl Psychiatry 5, e562.

Application References

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