Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb #9323

Glycogen synthase kinase   GSK-3   GSK-3beta   p-gsk3-b  

No. Size Price
9323S 100 µl ( 10 western blots ) ¥3,800.00 现货查询 购买询价
9323T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9323 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 46 Rabbit IgG
IHC-P 1:50
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb detects endogenous levels of GSK-3β only when phosphorylated at Ser9. The antibody may cross-react weakly with the phosphorylated form of GSK-3α due to high sequence homology.

Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗可以检测丝氨酸9位点发生磷酸化GSK-3β的水平 。由于具有高度同源性,此抗体可能与GSK-3α的磷酸化形式发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3β.

单克隆抗体是采用合成的与人源GSK-3 β蛋白丝氨酸9位点周围序列相对应的磷酸肽免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, λ-phosphatase- or PDGF-treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Western blot 分析NIH/3T3 细胞, λ磷酸酶和PDGF处理组,所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗 (上) 或GSK-3β (27C10) Rabbit mAb兔单抗 #9315 (下)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK3β (Ser9) (5B3) Rabbit mAb.

免疫组化分析石蜡包埋的人乳腺癌细胞,所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded wild type (left) or GSK-3β (-/-) MEFs, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb. (MEF wild type, GSK-3α knock out and GSK-3β knock out cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).免疫组化分析石蜡包埋的野生型或者GSK-3β (-/-) MEFs细胞,所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb。)。(MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞是由加拿大多伦多大学的Jim Woodgett博士友情提供。)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb on SignalSlide(TM) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)).

免疫组化分析SignalSlide(TM) Phospho-Akt (Ser473) IHC 对照#8101(分别为未处理(左)或者 LY294002处理的石蜡包埋的LNCaP细胞),使用的抗体是Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of wild type (left), GSK-3α (-/-) (center), or GSK-3β (-/-) (right) mouse embryonic fibroblasts (MEFs), FGF-treated (top) or phosphatase-treated (bottom), using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

免疫共聚焦分析分析野生型(左),GSK-3α (-/-) (中) 与 GSK-3β (-/-) (右) 的小鼠胚胎成纤维细胞(MEFs),并分为FGF 处理(上)和phosphatase处理组(下),使用的抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (绿色)。肌动蛋白纤维被DY-554环肽所染色(红色)。Blue pseudocolor为 DRAQ5® #4084 ( DNA染料的荧光)。(MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞由加拿大多伦多大学的Jim Woodgett博士友情提供。)

Western Blotting

Western Blotting

Western blot analysis of extracts from wild type (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and GSK-3β (-/-) (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or PDGF treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (upper) and GSK-3α/β Antibody (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Western blot分析野生型(通道1和2),GSK-3α (-/-) (通道 3和4) 与 GSK-3β (-/-) (通道5和6) 的小鼠胚胎成纤维细胞,并分为未处理和PDGF处理组,使用的抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗 (上) 和 GSK-3α/β Antibody (下)。(MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞是由加拿大多伦多大学的Jim Woodgett博士友情提供。)

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节Cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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