Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Tyk2 (Tyr1054/1055) Antibody #9321

MADR1   p135tyk2  

No. Size Price
9321S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9321 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 134 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Tyk2 (Tyr1054/1055) Antibody detects endogenous levels of Tyk2 only when phosphorylated at tyrosine1054/1055. The antibody does not cross-react with Tyk2 phosphorylated at other sites.

Phospho-Tyk2 (Tyr1054/1055) Antibody能够检测内源在tyrosine1054/1055位点磷酸化的Tyk2蛋白,抗体不与其它位点磷酸化的Tyk2蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1054/1055 of human Tyk2. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是通过合成对应人源性Tyk2 Tyr1054/1055 位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or interferon-alpha treated for the indicated times, using Phospho-Tyk2 (Tyr1054/1055) Antibody (upper) or Tyk2 antibody (lower).Western免疫印迹。用Phospho-Tyk2 (Tyr1054/1055) Antibody (上图) or Tyk2 antibody (下图)抗体研究未经处理或经干扰素—alpha处理一定时间的HeLa 细胞的细胞提取液。


Tyk2, a member of the Jak family of protein tyrosine kinases, associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and interferon-alpha and beta (1-3). Following ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-beta signaling cascade (5).

Tyk2是Jak酪氨酸蛋白激酶家族的一员,与很多细胞因子的受体有关并受到他们的激活,包括 IL-13, IL-6 家族, IL-10, 干扰素-alpha 和干扰素- beta (1-3)。 配体结合后,Tyk2 通过Tyr1054 和/或者 Tyr1055位点的磷酸化而激活 (4)。在IFN-beta信号级联反应中,Tyk2是Stat3的酪氨酸磷酸化所必需的(5)。

  1. Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-34.
  2. Stahl, N. et al. (1994) Science 263, 92-5.
  3. Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  4. Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-500.
  5. Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-11.

Application References

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