Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316

EC   Glycogen synthase kinase-3 alpha   GSK   GSK-3 alpha   GSK3A  

No. Size Price
9316L 300 µl ( 30 western blots ) ¥9,380.00 现货查询 购买询价
9316S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
9316T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9316 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 51 Rabbit
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb detects endogenous levels of GSK-3α when phosphorylated at Ser21 and does not detect GSK-3β when phosphorylated at Ser9.

Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb可以检测Ser21位点发生磷酸化的内源性GSK-3 α ,不能检测Ser9位点发生磷酸化的GSK-3β 。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser21 of human GSK-3α.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb (upper) or GSK-3α Antibody #9338 (lower).

Western blot 分析COS-7 细胞, λ磷酸酶和PDGF处理组,所用抗体为Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb兔单抗 (上) 或 GSK-3α Antibody #9338(下)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

免疫组化分析石蜡包埋的LNCaP细胞,分为未处理组(左)和LY294002处理组(右),所用抗体为Phospho-GSK-3α (Ser 21) (36E9) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma , untreated (left) or lambda phosphatase treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

免疫组化分析石蜡包埋的人乳腺癌肿瘤,分为未处理组(左)和λ磷酸酶处理组(右),所用抗体为Phospho-GSK-3α (Ser 21) (36E9) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

免疫组化分析石蜡包埋的人肺肿瘤,所用抗体为Phospho-GSK-3α (Ser 21) (36E9) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb in the presence of control peptide (left) or Phospho-GSK-3α (Ser21) (36E9) Blocking Peptide #1027 (right).

免疫组化分析石蜡包埋的人乳腺癌,所用抗体为在对照肽段下的Phospho-GSK-3α (Ser 21) (36E9) Rabbit mAb兔单抗(左)或者Phospho-GSK-3α (Ser21) (36E9) Blocking Peptide封闭肽 #1027(右)。


Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节Cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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