Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314

stat-5a   stat5 a   stat5 b  

No. Size Price
9314S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9314 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 90 Rabbit IgG
IHC-P 1:400
F 1:200
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine,

Specificity / Sensitivity

Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.

Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb能检测内源水平在Tyr694位点磷酸化的Stat5a蛋白和在Tyr699位点磷酸化的Stat5b蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr694 of Stat5.

此单克隆抗体是通过合成对应Stat5 Tyr694 位点周围的多肽免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from K562, untreated or treated with Gleevec for 1 hour, using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (upper) or Stat5 Antibody #9310 (lower).Western免疫印迹。Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (上图) or Stat5 Antibody #9310 (下图)研究未经处理和经过Gleevec处理1小时的K562细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells, Gleevec-treated (blue) or untreated (green), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.流式细胞仪研究未经处理(绿色)或经过Gleevec处理(蓝色) 的K562 细胞。所用抗体为Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, IFNα-treated (left) or untreated (right), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red).共聚焦免疫荧光。Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (绿色) and Pan-Keratin (C11) Mouse mAb #4545 (红色)研究IFNα处理(左图) 或者 未经处理 (右图)的HeLa。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded K562 cell pellets control (left) or STI-571-treated (right) using Phospho-Stat5 (Tyr694) Rabbit mAb.免疫组织化学染色分析石蜡包埋 K562 细胞沉淀(左图) 或者STI-571处理的K562 细胞沉淀(右图)。所用抗体为Phospho-Stat5 (Tyr694) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right).

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).

Stat5的激活是通过一系列的配体的结合而完成,包括 IL-2, GM-CSF,生长激素和泌乳刺激素。磷酸化Tyr694位点对 Stat5的活性是必须的(1,2)。此位点的磷酸化是在红细胞生成素的作用下由Src介导而完成(3)。 Stat5在某些白血病类型的细胞中有组成性的活性(4)。用IL-3处理的内皮细胞中有磷酸化的Stat5,这就表明它参与了血管再生和细胞的运动(5)。在不同细胞类型中 Stat5a 和 Stat5b 是被独立调控和激活的。例如,干扰素处理后在U-937细胞中Stat5a的活性较强但在HeLa细胞中Stat5b的活性较强(6)。

  1. Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
  2. Wakao, H. et al. (1994) EMBO J 13, 2182-91.
  3. Okutani, Y. et al. (2001) Oncogene 20, 6643-50.
  4. Demoulin, J.B. et al. (1999) J Biol Chem 274, 25855-61.
  5. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
  6. Meinke, A. et al. (1996) Mol Cell Biol 16, 6937-44.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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