Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Bad (Ser155) Antibody #9297

No. Size Price
9297S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9297T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9297 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Transfected Only 23 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Human, Rat,

Specificity / Sensitivity

Phospho-Bad (Ser155) Antibody detects transfected levels of Bad only when phosphorylated at Ser155. This antibody does not detect Bad phosphorylated at other sites or related family members.

Phospho-Bad (Ser155) Antibody只能检测转染的Ser155位点磷酸化的Bad 蛋白。此抗体不能检测在其它位点磷酸化的Bad,也不能检测其它相关家族成员。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser155 of mouse Bad. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的小鼠 Bad 蛋白Ser155 周围残基相对应的磷磷化肽段免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of cell extracts from 293 cells transfected with Wild-type Bad, Bad (S112A/S136A), Bad (S155A) and treated with forskolin (30 µM), using Phospho-Bad (Ser155) Antibody (upper) and Bad Antibody #9292 (lower).

Western blot 免疫印记分析转染了野生型Bad、Bad (S112A/S136A)、Bad (S155A) 并经过forskolin (30 µM) 处理的293细胞的细胞提取液。用Phospho-Bad (Ser155) Antibody (上图) 和Bad Antibody #9292 (下图)。


Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Bad是Bcl-2促凋亡家族中的成员,它通过转移 Bax 与 Bcl-2 和 Bcl-xL 结合而促进细胞死亡(1,2)。存活因子如IL-3, 能够通过激活细胞内信号通路,导致Bad在Ser112和Ser136位点发生磷酸化,进而抑制凋亡活性(2)。在这些位点的磷酸化促进Bad与 14-3-3 蛋白的结合,从而阻止了Bad与Bcl-2和Bcl-xl的互作(2)。Akt在Ser136位点磷酸化Bad,而促进细胞的存活(3,4)。Bad在体内和体外都能够被p90RSK(5,6)和线粒体锚定PKA磷酸化Ser112位点(7)。PKA对BH3结构域Ser155位点的磷酸化,在阻止Bad和Bcl-xL的二聚化过程中发挥了重要的作用(8-10)。

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-628.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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