Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Bad (Ser112) (7E11) Mouse mAb #9296

Bad   bcl   bcl2  

No. Size Price
9296S 200 µl ( 40 western blots ) ¥3,900.00 现货查询 购买询价
9296T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9296 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Monkey, Endogenous 23 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Bad (Ser112) (7E11) Mouse mAb detects endogenous levels of Bad only when phosphorylated at serine112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.

Phospho-Bad (Ser112) (7E11) Mouse mAb 鼠单抗只能检测内源的ser112位点磷酸化的Bad。Ser112系统命名法是建立在小鼠序列的基础上。同功蛋白磷酸化位点在人源蛋白中是Ser75 ,在大鼠中是Ser113 。次抗体不能检测在其它位点磷酸化的Bad,也不能检测其它相关家族的成员。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad.

该单克隆抗体是通过合成对应小鼠源Bad Ser112位点周围残基的磷酸化肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells untreated or TPA-treated, using Phospho-Bad (Ser112) (7E11) Mouse mAb (upper) or Bad Antibody #9292 (lower).

Western blot 免疫印记。用Phospho-Bad (Ser112) (7E11) Mouse mAb 鼠单抗(上图) 或者 Bad Antibody #9292 (下图)研究未经过处理或者经过TPA处理的 COS 细胞的细胞提取液。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A), Bad (S112A/S136A) and treated with TPA or forskolin, using Phospho-Bad (Ser112) (7E11) Mouse mAb (upper) or Bad Antibody #9292 (lower).

Western blot(免疫印迹)分析分别转染了野生型Bad、Bad (Ser112A)、Bad (S136A)、Bad (S112A/S136A)并用TPA或者forskolin处理的 293 细胞的细胞提取液,用Phospho-Bad (Ser112) (7E11) Mouse mAb 鼠单抗(上图) 或者Bad Antibody #9292 (下图)研究。


Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Bad是Bcl-2促凋亡家族中的成员,它通过转移 Bax 与 Bcl-2 和 Bcl-xL 结合而促进细胞死亡(1,2)。存活因子如IL-3, 能够通过激活细胞内信号通路,导致Bad在Ser112和Ser136位点发生磷酸化,进而抑制凋亡活性(2)。在这些位点的磷酸化促进Bad与 14-3-3 蛋白的结合,从而阻止了Bad与Bcl-2和Bcl-xl的互作(2)。Akt在Ser136位点磷酸化Bad,而促进细胞的存活(3,4)。Bad在体内和体外都能够被p90RSK(5,6)和线粒体锚定PKA磷酸化Ser112位点(7)。PKA对BH3结构域Ser155位点的磷酸化,在阻止Bad和Bcl-xL的二聚化过程中发挥了重要的作用(8-10)。

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-628.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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