Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Bad (Ser136) Antibody #9295

Bad  

No. Size Price
9295S 200 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9295 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:500 Mouse, Transfected Only 23 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Human, Rat,

Specificity / Sensitivity

Phospho-Bad (Ser136) Antibody detects transfected levels of Bad only when phosphorylated at Ser136. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.

Phospho-Bad (Ser136) Antibody 能够检测转染水平的只在 Ser136位点磷酸化的Bad。此抗体既不与在别的位点磷酸化的Bad交叉反应,也不与其它相关家族成员交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser136 of mouse Bad. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是通过合成小鼠源对应的Bad Ser136位点周围残基的磷酸化肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (S112A), Bad (S136A) or Bad (S112A/S136A), untreated, TPA-treated or forskolin-treated, using Phospho-Bad (Ser112) Antibody #9291 (top), Phospho-Bad (Ser136) Antibody (middle) or Bad Antibody #9292 (bottom).

Western blot(免疫印迹)分析分别转染了野生型Bad、Bad (Ser112A)、Bad (S136A)、Bad (S112A/S136A)并经TPA或者forskolin处理的或者未经处理的 293 细胞的细胞提取液,用Phospho-Bad (Ser112) Antibody #9291 (上图),Phospho-Bad (Ser136) Antibody (中间图) or Bad Antibody #9292 (下图)研究。

Western Blotting

Western Blotting

Western blot analysis of GST-Bad, phosphorylated by CKII or PKA in vitro, using Phospho-Bad (Ser112) Antibody #9291 (top), Phospho-Bad (Ser136) Antibody (middle) or Bad Antibody #9292 (bottom).

Western免疫印迹。用Phospho-Bad (Ser112) Antibody #9291 (上图), Phospho-Bad (Ser136) Antibody (中间图) or Bad Antibody #9292 (下图)研究被CKII或PKA体外磷酸化的GST-Bad蛋白。

Background

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Bad是Bcl-2促凋亡家族中的成员,它通过转移 Bax 与 Bcl-2 和 Bcl-xL 结合而促进细胞死亡(1,2)。存活因子如IL-3, 能够通过激活细胞内信号通路,导致Bad在Ser112和Ser136位点发生磷酸化,进而抑制凋亡活性(2)。在这些位点的磷酸化促进Bad与 14-3-3 蛋白的结合,从而阻止了Bad与Bcl-2和Bcl-xl的互作(2)。Akt在Ser136位点磷酸化Bad,而促进细胞的存活(3,4)。Bad在体内和体外都能够被p90RSK(5,6)和线粒体锚定PKA磷酸化Ser112位点(7)。PKA对BH3结构域Ser155位点的磷酸化,在阻止Bad和Bcl-xL的二聚化过程中发挥了重要的作用(8-10)。

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-628.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

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