Cell Signaling Technology

Product Pathways - Flow Cytometry

Phospho-Bad (Ser112) Antibody #9291

bcl   bcl2  

No. Size Price
9291L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
9291S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9291T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
9291 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 23 Rabbit
IP 1:50
F 1:100
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Bad (Ser112) Antibody detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.

Phospho-Bad (Ser112) Antibody 只能检测内源的在Ser112位点磷酸化的Bad。Ser112系统命名法是建立在小鼠序列的基础上。同功蛋白磷酸化位点在人源蛋白中是Ser75,在大鼠中是Ser113。此抗体不能检测在其它位点磷酸化的Bad,也不能检测其它相关家族的成员。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是通过合成对应小鼠源Bad Ser112位点周围残基的磷酸化肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A), Bad (S112A/S136A) and treated with TPA or forskolin, using Phospho-Bad (Ser112) Antibody (upper) or Bad Antibody #9292 (lower).

Western blot 免疫印迹分析分别转染了野生型Bad、Bad (Ser112A)、Bad (S136A)、Bad (S112A/S136A)并用TPA或者forskolin处理的 293 细胞的细胞提取液,所用抗体为Phospho-Bad (Ser112) Antibody (上图) 或者Bad Antibody #9292 (下图)研究。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Bad Antibody #9292 (right) or Phospho-Bad (Ser112) Antibody (left).

Western blot 免疫印迹分析未处理或者经TPA处理的COS 细胞的细胞提取液,使用抗体为 Bad Antibody #9292 (右图) 或者Phospho-Bad (Ser112) Antibody (左图)。

Western Blotting

Western Blotting

Western blot analysis of Bad fusion protein phosphorylated by CKII or PKA in vitro, using Phospho-Bad (Ser112) Antibody (upper) or Bad Antibody #9292 (lower).

Western blot 免疫印迹分析用CKII或者PKA体外处理的Bad蛋白的磷酸化,所用抗体为 Phospho-Bad (Ser112) Antibody (上图) 或者 Bad Antibody #9292 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells, untreated (blue) or Calyculin A/TPA-treated (green), usingPhospho-Bad (Ser112) Antibody compared to Rabbit (DA1E) mAb IgG Isotype Control #3900 (red).

流式细胞仪分析COS细胞,未经处理(蓝色)或经过经 Calyculin A/TPA 处理(绿色),所用抗体为Phospho-Bad (Ser112) Antibody 与非特异性Rabbit (DA1E) mAb IgG Isotype Control #3900 (红色)做比对。


Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Bad是Bcl-2促凋亡家族中的成员,它通过转移 Bax 与 Bcl-2 和 Bcl-xL 结合而促进细胞死亡(1,2)。存活因子如IL-3, 能够通过激活细胞内信号通路,导致Bad在Ser112和Ser136位点发生磷酸化,进而抑制凋亡活性(2)。在这些位点的磷酸化促进Bad与 14-3-3 蛋白的结合,从而阻止了Bad与Bcl-2和Bcl-xl的互作(2)。Akt在Ser136位点磷酸化Bad,而促进细胞的存活(3,4)。Bad在体内和体外都能够被p90RSK(5,6)和线粒体锚定PKA磷酸化Ser112位点(7)。PKA对BH3结构域Ser155位点的磷酸化,在阻止Bad和Bcl-xL的二聚化过程中发挥了重要的作用(8-10)。

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-628.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

Application References

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