Cell Signaling Technology

Product Pathways - MAPK Signaling

c-Jun Control Cell Extracts #9263

No. Size Price
9263S 150 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9263 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated c-Jun (Ser63) II Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer. 
 
 Phosphorylated c-Jun (Ser63) II Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared with UV treatment, serve as a positive control. Supplied in SDS Sample Buffer.

非磷酸化的c-Jun (Ser63) II Cell Extracts:未经处理的NIH/3T3细胞总提取物,作为阴性对照。加入SDS样品缓冲液。磷酸化的c-Jun (Ser63) II Cell Extracts:经紫外照射的NIH/3T3细胞总提取物,作为阳性对照。加入SDS样品缓冲液。

Western Blotting

Western Blotting

Western blot analysis of c-Jun Control Cell Extracts using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270 (upper) and c-Jun (60A8) Rabbit mAb #9165 (lower).

Applications

As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated c-Jun control extracts.

作为对照使用时,我们建议用15 µl磷酸化和非磷酸化的c-Jun蛋白对照提取物。

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

c-Jun是Jun家族的一个成员,Jun家族成员包括c-Jun, JunB, 和 JunD。c-Jun是转录因子激活蛋白-1(AP-1)的一个组分。AP-1是由Fos和Jun形成的二聚体以及 ATF家族成员组成的,能够结合并激活TRE/AP-1元件的转录(1)。包括生长因子,细胞因子和压力在内的细胞外信号能够激活AP-1依赖的转录过程。C-Jun的转录活性是通过SAPK/JNK对其中的Ser63和Ser73的磷酸化来调控的(2)。小鼠敲除实验表明c-Jun对于胚胎形成是至关重要的(3),进一步的研究发现c-Jun在多种组织和发育过程中发挥作用,包括轴突再生(4),肝脏再生(5)和T细胞发育(6)。AP-1所调控的基因发挥了多种生物学功能,包括细胞增殖、分化、凋亡、转化,侵润和转移,这依赖于细胞种类和具体情况(7-9)。其他的AP-1所调控的靶基因还能够调节细胞存亡,以及低氧和血管生成(8,10)。研究提示c-Jun可以作为癌症、血管重构、急性炎症反应和风湿性关节炎的重要治疗靶点(11,12)。

  1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
  2. Davis, R.J. (2000) Cell 103, 239-52.
  3. Hilberg, F. et al. (1993) Nature 365, 179-81.
  4. Raivich, G. et al. (2004) Neuron 43, 57-67.
  5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
  6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
  7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
  9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
  10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
  11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
  12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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