Cell Signaling Technology

Product Pathways - MAPK Signaling

SAPK/JNK Control Cell Extracts #9253

No. Size Price
9253S 150 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9253 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated SAPK/JNK Control Cell Extracts: Total cell extracts from 293 cells, prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer. 
 
 
 Phosphorylated SAPK/JNK Control Cell Extracts: Total cell extracts from 293 cells, treated with UV light, serve as a positive control. Supplied in SDS Sample Buffer.

非磷酸化的SAPK/JNK Control Cell Extracts:未经处理的 293细胞总提取物,作为阴性对照。加入SDS样品缓冲液。磷酸化的SAPK/JNK Control Cell Extracts:紫外照射的293细胞总提取物,作为阳性对照。加入SDS样品缓冲液。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, treated with or without UV light, using Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb (upper) and SAPK/JNK (56G8) Rabbit mAb (lower).

Applications

As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated SAPK/JNK control extracts. Boil 2 minutes prior to use.

作为对照,我们建议用15 µl磷酸化和非磷酸化的SAPK/JNK蛋白对照提取物。

Background

应激活化蛋白激酶/Jun氨基末端激酶SAPK/JNK能够有效、优先地被多种环境压力激活,这些环境压力包括紫外照射和gamma 辐射、神经酰胺、炎性因子,在某些情况下也包括生长因子和GPCR激动剂 (1-6)。正如其他的 MAPKs,核心信号传导单元包括一个MAPKKK,通常是MEKK1-MEKK4,或一种混合谱系酶 (MLKs),这些MAPKKK激酶能磷酸化并激活 MKK4/7。活化后,MKKs磷酸化并激活SAPK/JNK 激酶 (2)。应激反应信号经Rho 家族(Rac, Rho, cdc42)的小GTP酶被传递到这个级联信号反应中 (3)。Rac1 和 cdc42蛋白都能调节MEKKs、 MLKs的激活(3)。另外一种情况是,MKK4/7能够通过生发中心激酶 (GCK)家族成员的刺激,在一个非GTP酶依赖机制中被激活 (4)。有三种不同的SAPK/JNK基因,而每一种都会经过选择性剪切产生多种异构体(3)。活化的二聚体SAPK/JNK,能转位到细胞核中,通过它对 c-Jun, ATF-2和其他转录因子的效应而调节转录过程(3,5)。

  1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
  2. Ichijo, H. (1999) Oncogene 18, 6087-93.
  3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
  4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
  5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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