Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246

i kappa b   IκB-α   IκBα   MAD3  

No. Size Price
9246L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9246S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
9246 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Guinea Pig,

Specificity / Sensitivity

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb detects endogenous levels of IκBα only when phosphorylated at Ser32/36.

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb抗体只能检测内源的在Ser32/36 位点磷酸化的IκBα蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα.

单克隆是通过合成人源的对应的IκBα Ser32/36位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated orTNF-α-treated (#2169, 20 ng/ml) for 5 minutes, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) or IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).Western免疫印迹。用Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (上图) 或者 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (下图)研究未经处理或经TNF-α处理 (#2169, 20 ng/ml,5 min) 的NIH/3T3 细胞的细胞提取液。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24 h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) and IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).Western免疫印迹。用Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (上图) 和 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (下图).研究经TPA (#9905, 80 nM for 24 h)分化和 1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。磷酸化IκBα 的Ser32 和 Ser36位点后,蛋白水解酶体介导的降解使得 NF-κB释放激活并入核(3-7)。IκBα的磷酸化导致了Rel依赖的转录能够被很多胞外信号激活,包括炎症因子,生长因子和趋化因子 。能够磷酸化IκB这些活性位点的激酶已经得到了鉴定(8)。

  1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
  2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
  3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
  4. Brown, K. et al. (1995) Science 267, 1485-8.
  5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications