Cell Signaling Technology

Product Pathways - NF-kB Signaling

NF-κB Control Cell Extracts #9243

cell extracts   control extracts   extracts   hela   ikappab   ikbb   ikk   IκB-α   iκbα   nf-kappab   nf-kb   nfkb   rel   tnf  

No. Size Price
9243S 200 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9243 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Untreated NF-κB Control Cell Extracts: 
 Total cell extracts from HeLa cells prepared without treatment serve as a negative control. Supplied in blue SDS Sample Buffer. 
 TNF-treated NF-κB Control Cell Extracts: 
 Total cell extracts from HeLa cells prepared with Human Tumor Necrosis Factor-α (hTNF-α) #8902 treatment serve as a positive control. Supplied in red SDS Sample Buffer.Untreated NF-κB Control Cell Extracts: 


未经处理的HeLa细胞的总细胞提取液作为阴性对照。保存在blue SDS样品缓冲液中。 TNF-treated NF-κB Control Cell Extracts: 
经过肿瘤坏死因子(hTNF-α) #8902 处理的 HeLa细胞的总细胞提取液作为阳性对照。保存在红色的SDS样品缓冲液中。

Western Blotting

Western Blotting

Western blot analysis of NF-κB Control Cell Extracts #9243, using IκBα (44D4) Rabbit mAb #4812 (left) and Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (right).

Western Blotting

Western Blotting

Western blot analysis of NF-κB Control Cell Extracts #9243, using IKKα Antibody #2682 (upper), IKKβ (2C8) Rabbit mAb #2370 (middle) and Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 (lower).

Western Blotting

Western Blotting

Western blot analysis of NF-κB Control Cell Extracts #9243, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (left) and NF-κB p65 (E498) Antibody #3987 (right).

Applications

Western Blots: CST recommends using 20 µl of untreated and TNF-treated NF-κB cell extracts as controls.

Western blot。CST推荐用20 µl未处理的和经TNF处理的NF-κB 细胞提取液作为对照。

Background

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

核因子κ B(NF-κB)/Rel 家族的转录调控因子在炎症反应和免疫反应中发挥了至关重要的作用(1,2)。在哺乳动物中一共有5个家族成员: RelA, c-Rel, RelB, NF-κB1 (p105/p50) 和 NF-κB2 (p100/p52)。 p105 和 p100 在蛋白水解酶的作用下分别形成p50 和 p52。Rel与p50和p52形成二聚体,此复合体能够结合到DNA上调控转录。在未刺激的状态下, NF-κB 在IκB抑制剂作用下在细胞质中处于非活性状态(3-5)。 NF-κB激活因子能够诱导 IκB 蛋白的磷酸化, 这就使IκB能够快速的经过泛素化-蛋白酶通路降解从而释放 NF-κB,激活的NF-κB入核调控基因的表达(6-8)。 NIK 和 IKKα (IKK1) 调节磷酸化并促使NF-κB2 (p100) 生成p52, p52之后会入核发挥功能(9-11)。

  1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
  2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
  3. Haskill, S. et al. (1991) Cell 65, 1281-9.
  4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
  5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
  8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  9. Senftleben, U. et al. (2001) Science 293, 1495-9.
  10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
  11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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