Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb #9236

MAPK/ERK-Kinase3   MAPK/ERK-Kinase6   MAPKK3   MAPKK6   MEK3   MEK6   mkk346   rabbit-monoclonal   SAPKK3  

No. Size Price
9236L 300 µl ( 30 western blots ) ¥9,325.00 现货查询 购买询价
9236S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9236 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 P-MKK3, 41 P-MKK6 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb detects endogenous levels of MKK3/MKK6 only when phosphorylated at serine189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1.

Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb兔单抗能够识别内源性Ser189/207位点磷酸化的MKK3/MKK6蛋白水平。该抗体不能识别MEK1, MEK2和MKK4/SEK1蛋白的相应磷酸化残基。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser189/207 of human MKK3.

该单克隆抗体是采用合成的与人源MKK3蛋白Ser189/207 位点周围残基相一致的磷酸化肽段免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or UV-treated COS, NIH/3T3 and C6 cells, using Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb.Western blot 方法检测细胞提取物:未经处理的和紫外照射的COS、C6、NIH/3T3细胞,使用的抗体是 Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb。

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).

MKK3和MKK6是两种密切相关的双特异性蛋白激酶,能够激活p38 MAPK(1-5)。MKK3和MKK6能够通过磷酸化p38 MAPK的活化位点Thr-Gly-Tyr从而使p38 MAPK激活,但不能磷酸化或激活Erk1/2或SAPK/JNK。p38 MAPK的磷酸化能够显著提高其磷酸化蛋白底物的能力,这些底物包括ATF-2和Elk-1。MKK3和MKK6能被不同形式的细胞压力和炎症细胞因子激活(4,5)。通过磷酸化MKK3的Ser189和Thr193位点(2)以及MKK6的Ser207和Thr211位点(4,5)可以激活MKK3和MKK6。

  1. Derijard, B. et al. (1995) Science 267, 682-685.
  2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
  4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
  5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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