Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231

MAPK/ERK Kinase3   MAPK/ERK Kinase6   MAPKK3   MAPKK6   MEK3   MEK6   mkk346   SAPKK3  

No. Size Price
9231S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9231 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 40 Phospho-MKK3. 41 Phospho-MKK6. Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MKK3/MKK6 (Ser189/207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1.

Phospho-MKK3/MKK6 (Ser189/207) Antibody兔多抗能检测内源性Ser189/207位点磷酸化的MKK3/MKK6蛋白水平。该抗体不能识别MEK1, MEK2和MKK4/SEK1蛋白的相应磷酸化残基。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源MKK3蛋白Ser189/207位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or anisomycin-treated for the indicated times, using Phospho-MKK3/MKK6 (Ser189/207) Antibody (upper) or MKK3 Antibody #9232 (lower).Western blot 方法检测细胞提取物:未经处理的和用茴香霉素按规定时间处理的、NIH/3T3细胞,使用的抗体是Phospho-MKK3/MKK6 (Ser189/207) Antibody (上图)和MKK3 Antibody #9232 (下图)。

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).

MKK3和MKK6是两种密切相关的双特异性蛋白激酶,能够激活p38 MAPK(1-5)。MKK3和MKK6能够通过磷酸化p38 MAPK的活化位点Thr-Gly-Tyr从而使p38 MAPK激活,但不能磷酸化或激活Erk1/2或SAPK/JNK。p38 MAPK的磷酸化能够显著提高其磷酸化蛋白底物的能力,这些底物包括ATF-2和Elk-1。MKK3和MKK6能被不同形式的细胞压力和炎症细胞因子激活(4,5)。通过磷酸化MKK3的Ser189和Thr193位点(2)以及MKK6的Ser207和Thr211位点(4,5)可以激活MKK3和MKK6。

  1. Derijard, B. et al. (1995) Science 267, 682-685.
  2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
  4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
  5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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