Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-ATF-2 (Thr69/71) Antibody #9225

Activating Transcription Factor 2   atf 2   ATF2   CRE-BP1   CREB2   phospho-atf  

No. Size Price
9225S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9225 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70 Rabbit
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-ATF-2 (Thr69/71) Antibody detects endogenous levels of ATF-2 only when dually phosphorylated at both threonine 69 and threonine 71. It does not recognize ATF-2 singly phosphorylated at either threonine 69 or threonine 71.

Phospho-ATF-2 (Thr69/71) Antibody兔多抗能够检测内源性的Thr69和Thr71位点共磷酸化的ATF-2蛋白水平,但是不能识别Thr69位点或Thr71位点单独磷酸化的ATF-2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr69 and Thr71 of human ATF-2. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源ATF-2蛋白Thr69和Thr71位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or UV-treated (lanes 1 and 2), NIH/3T3 cells, untreated or anisomycin-treated (lanes 3 and 4), and C6 cells, untreated or anisomycin-treated (lanes 5 and 6), using Phospho-ATF-2 (Thr69/71) Antibody.western blot方法检测细胞提取物:未经处理和紫外照射(1、2号条带)的 293 细胞,未处理和茴香霉素处理的(3、4号条带)NIH/3T3 细胞,未处理和茴香霉素处理的(5、6号条带)C6细胞。使用的抗体是Phospho-ATF-2 (Thr69/71) Antibody。

Table

Table

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-ATF-2 (Thr69/71) Antibody.免疫组织化学方法检测石蜡包埋的人乳腺癌组织,显示出细胞核的位置。使用的抗体是Phospho-ATF2 (Thr69/71) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin treated (right), using Phospho-ATF-2 (Thr69/71) Antibody.免疫组织化学方法检测石蜡包埋的 NIH/3T3细胞,左侧表示未经处理的细胞,右侧表示经茴香霉素处理的细胞。使用的抗体是Phospho-ATF2 (Thr69/71) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcioma, control (left) or lambda phosphatase-treated (right), using Phopsho-ATF2 (Thr69/71) Antibody.免疫组织化学方法检测石蜡包埋的人肺癌组织,左侧是对照组,右侧是用lambda磷酸化后的组织。使用的抗体是Phospho-ATF2 (Thr69/71) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcioma using Phospho-ATF2 (Thr69/71) Antibody.免疫组织化学方法检测石蜡包埋的人结肠癌组织,使用的抗体是Phospho-ATF2 (Thr69/71) Antibody。

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

转录因子ATF-2(也被称为 CRE-BP1)能够结合到AP-1和 CRE DNA反应元件,是ATF/CREB亮氨酸拉链蛋白质家族的成员(1)。ATF-2蛋白能够调控多种病毒癌基因以及抑癌基因的表达,是SAPK/JNK 、p38 MAPK信号传导通路的靶标 (2-4)。多种细胞压力包括基因毒剂、炎性细胞因子和紫外辐射,都能激活ATF-2蛋白的转录活性。细胞应激刺激通过磷酸化Thr69和Thr71位点使ATF-2蛋白被激活 (2-4)。在体外以及转染了ATF-2的细胞中,SAPK、p38 MAPK蛋白都被证明能够磷酸化ATF-2蛋白的这些位点。这些位点的突变能导致应激性的ATF-2诱导转录活性降低(2-4),并且降低E1A 、Rb蛋白通过ATF-2介导的基因表达。(2)。

  1. Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
  2. Gupta, S. et al. (1995) Science 267, 389-93.
  3. van Dam, H. et al. (1995) EMBO J 14, 1798-811.
  4. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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