Cell Signaling Technology

Product Pathways - MAPK Signaling

ATF-2 Control Cell Extracts #9223

Activating Transcription Factor 2   atf 2   ATF2   control cell   control cell extract   extract   lysate   phospho-atf  

No. Size Price
9223S 200 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9223 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


Nonphosphorylated ATF-2 Control Cell Extracts: Total extracts from NIH/3T3 cells, prepared without treatment, to serve as a negative control.. Supplied in SDS Sample Buffer. Store at -20ºC. 
 Phosphorylated ATF-2 Control Cell Extracts: Total extracts from NIH/3T3 cells, prepared with anisomycin treatment, to serve as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.

非磷酸化的ATF-2蛋白对照细胞提取物:未经处理的 NIH/3T3细胞总提取物,作为阴性对照。以SDS样品缓冲液的形式供应,-20ºC保存。磷酸化的ATF-2蛋白对照细胞提取物:茴香霉素处理的 NIH/3T3细胞总提取物,作为阳性对照。以SDS样品缓冲液的形式供应,-20ºC保存。

Western Blotting

Western Blotting

Western blot analysis of ATF-2 Control Cell Extracts using Phospho-ATF-2 (Thr69/71) Antibody #9225 (upper) and ATF-2 (20F1) Rabbit mAb #9226 (lower).


Western Blots: For controls, we recommend using 20 µl of phosphorylated and nonphosphorylated ATF-2 control extracts.

免疫印记法:作为对照,我们建议使用20 µl磷酸化和非磷酸化的ATF-2对照物。


The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

转录因子ATF-2(也被称为 CRE-BP1)能够结合到AP-1和 CRE DNA反应元件,是ATF/CREB亮氨酸拉链蛋白质家族的成员(1)。ATF-2蛋白能够调控多种病毒癌基因以及抑癌基因的表达,是SAPK/JNK 、p38 MAPK信号传导通路的靶标 (2-4)。多种细胞压力包括基因毒剂、炎性细胞因子和紫外辐射,都能激活ATF-2蛋白的转录活性。细胞应激刺激通过磷酸化Thr69和Thr71位点使ATF-2蛋白被激活 (2-4)。在体外以及转染了ATF-2的细胞中,SAPK、p38 MAPK蛋白都被证明能够磷酸化ATF-2蛋白的这些位点。这些位点的突变能导致应激性的ATF-2诱导转录活性降低(2-4),并且降低E1A 、Rb蛋白通过ATF-2介导的基因表达。(2)。

  1. Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
  2. Gupta, S. et al. (1995) Science 267, 389-93.
  3. van Dam, H. et al. (1995) EMBO J 14, 1798-811.
  4. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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