Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-ATF-2 (Thr71) Antibody #9221

Activating Transcription Factor 2   atf 2   ATF2   CRE-BP1   CREB2   phospho-atf  

No. Size Price
9221L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9221S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9221 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70 Rabbit
IP 1:250
IHC-P 1:50
F 1:50
IF-IC 1:50
IHC-F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),

Specificity / Sensitivity

Phospho-ATF-2 (Thr71) Antibody detects endogenous levels of ATF-2 only when phosphorylated at threonine 71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well.

Phospho-ATF-2 (Thr71) Antibody 兔多抗能够检测Thr71位点磷酸化的内源性ATF-2蛋白水平,不能与磷酸化的c-Jun, CREB蛋白和其他转录因子发生交叉反应。它能识别Thr69和Thr71位点双磷酸化和Thr71位点单磷酸化的ATF-2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源ATF-2蛋白Thr71位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-ATF-2 (Thr71) Antibody .免疫组织化学方法检测石蜡包埋的人乳腺癌组织,显示出细胞核的位置。使用的抗体是Phospho-ATF-2 (Thr71) Antibody

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistchemical analysis of paraffin-embedded human colon carcinoma using Phospho-ATF-2 (Thr71) Antibody.免疫组织化学方法检测石蜡包埋的人结肠癌组织,使用的抗体是Phospho-ATF-2 (Thr71) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-ATF-2 (Thr71) Antibody.免疫组织化学方法检测石蜡包埋的人肺癌组织,使用的抗体是Phospho-ATF-2 (Thr71) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody.Western blot 方法检测细胞提取物:未经处理的和茴香霉素处理的、HeLa、C6、NIH/3T3细胞,使用的抗体是 Phospho-ATF-2 (Thr71) Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-ATF-2 (Thr71) Antibody compared to a nonspecific negative control antibody (red).Flow Cytometry方法检测细胞提取物:未经处理(蓝色)的和茴香霉素处理的(绿色)、 Jurkat 细胞,使用的抗体是 Phospho-ATF-2 (Thr71) Antibody,红色表示非特异性阴性对照抗体。

IHC-F (frozen)

IHC-F (frozen)

Immunhistochemical analysis of frozen H1650 xenograft using Phospho-ATF-2 (Thr71) Antibody.免疫组织化学方法检测石蜡包埋的冻存 H1650移植瘤组织,使用的抗体是Phospho-ATF-2 (Thr71) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, either untreated (left) or anisomycin-treated (right), using Phospho-ATF-2 (Thr71) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测:未经处理的(左侧)或茴香霉素处理的(右侧)HeLa 细胞,使用的抗体是Phospho-ATF-2 (Thr71) Antibody (绿色),肌动蛋白纤维素已被 Alexa Fluor® 555 phalloidin (红色)标记。

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

转录因子ATF-2(也被称为 CRE-BP1)能够结合到AP-1和 CRE DNA反应元件,是ATF/CREB亮氨酸拉链蛋白质家族的成员(1)。ATF-2蛋白能够调控多种病毒癌基因以及抑癌基因的表达,是SAPK/JNK 、p38 MAPK信号传导通路的靶标 (2-4)。多种细胞压力包括基因毒剂、炎性细胞因子和紫外辐射,都能激活ATF-2蛋白的转录活性。细胞应激刺激通过磷酸化Thr69和Thr71位点使ATF-2蛋白被激活 (2-4)。在体外以及转染了ATF-2的细胞中,SAPK、p38 MAPK蛋白都被证明能够磷酸化ATF-2蛋白的这些位点。这些位点的突变能导致应激性的ATF-2诱导转录活性降低(2-4),并且降低E1A 、Rb蛋白通过ATF-2介导的基因表达。(2)。

  1. Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
  2. Gupta, S. et al. (1995) Science 267, 389-93.
  3. van Dam, H. et al. (1995) EMBO J 14, 1798-811.
  4. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

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