Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215

CSBP   HOGK   p38   p38-MAP-kinase   p38-MAPK   p38-β   p38-γ   p38-δ   p38β   p38γ   p38δ   RK   SAPK-2   sc-7973  

No. Size Price
9215L 600 µl ( 60 western blots ) ¥9,325.00 现货查询 购买询价
9215S 200 µl ( 20 western blots ) ¥3,780.00 现货查询 购买询价
9215 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster,Pig,S. cerevisiae, Endogenous 43 Rabbit IgG
F 1:25
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Hamster, Mink, Zebrafish, Bovine,

Specificity / Sensitivity

Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb detects endogenous levels of p38 MAPK only when dually phosphorylated at Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK.

Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb兔单抗能够检测内源性Thr180位点和Tyr182位点共磷酸化的p38 MAPK蛋白水平。该抗体不能与p42/44 MAPK和SAPK/JNK的相关磷酸化形式发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK.

该单克隆抗体是采用合成的与人源p38 MAPK蛋白Thr180/Tyr182位点周围残基相一致的磷酸化肽段免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, C6, NIH/3T3 and COS cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (upper) or p38 MAPK Antibody #9212 (lower).western blot方法检测细胞提取物:未处理和按规定处理的Jurkat, C6, NIH/3T3、COS,使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (上图)和 p38 MAPK Antibody #9212 (下图)。

Western Blotting

Western Blotting

Specificity of Phospho-Erk1/2, Phospho-p38 MAPK and Phospho-SAPK/JNK Rabbit mAb: Western blot analysis of extracts from NIH/3T3 cells treated with PDGF and UV, using Phospho-p38 MAPK Rabbit mAb #9215, Phospho-SAPK/JNK Rabbit mAb and Phospho-Erk1/2 Rabbit mAb.Phospho-Erk1/2, Phospho-p38 MAPK和Phospho-SAPK/JNK Rabbit mAb特异性分析western blot方法检测PDGF 、UV处理的NIH/3T3细胞提取物,使用的抗体是Phospho-p38 MAPK Rabbit mAb #9215, Phospho-SAPK/JNK Rabbit mAb和Phospho-Erk1/2 Rabbit mAb.。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物:未经处理(蓝色)和茴香霉素处理的(绿色)Jurkat细胞,使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mA,红色曲线表示非特异性阴性对照抗体。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182)(3D7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞,左侧是未经处理的样品,右侧是经茴香霉素处理的样品,使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182)(3D7) Rabbit mAb(呈绿色)。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记,呈红色。

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). 
 SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

p38 MAP kinase (MAPK),也被称为RK (1)或CSBP (2),是酵母中HOG激酶在哺乳动物中的同源蛋白,在细胞因子和应急诱导的信号转导过程中起重要作用。(1-4)。已经发现了4种亚型的p38 MAPK,分别是p38α, β, γ(也被称为Erk6或 SAPK3)以及δ (也被称为 SAPK4)。与SAPK/JNK通路类似,p38 MAPK可以被多种细胞压力所激活,包括渗透压休克,炎症因子,脂多糖(LPS),紫外线照射和生长因子(1-5)。MKK3, MKK6和SEK能够使p38 MAPK的Thr180 和Tyr182位点磷酸化,从而激活p38 MAPK。激活的p38 MAPK能够磷酸化并激活MAPKAP kinase 2 (3),进而最终磷酸化ATF-2 (5), Max (6)和MEF2等转录因子(5-8)。SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole)是p38 MAPK的选择性抑制剂。该复合物通过抑制p38 MAPK及后续HSP27的磷酸化,抑制MAPKAPK-2的激活(9)。SB203580能够结合到p38 MAPK与ATP结合的位置从而抑制p38 MAPK的催化活性,但不会通过上游激酶抑制p38 MAPK的磷酸化(10)。

  1. Rouse, J. et al. (1994) Cell 78, 1027-37.
  2. Han, J. et al. (1994) Science 265, 808-11.
  3. Lee, J.C. et al. (1994) Nature 372, 739-46.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-49.
  5. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
  6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
  7. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.
  9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
  10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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