Cell Signaling Technology

Product Pathways - MAPK Signaling

p38 MAPK Control Cell Extracts #9213

CSBP   HOGK   p38   p38-MAP-kinase   p38-MAPK   p38-α   p38-β   p38-γ   p38-δ   p38α   p38β   p38γ   p38δ   RK   SAPK-2  

No. Size Price
9213S 150 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9213 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated p38 MAPK Control Cell Extracts: Total extracts from C-6 glioma cells prepared without anisomycin treatment to serve as a negative control. Supplied in SDS Sample Buffer. 
 Phosphorylated p38 MAPK Control Cell Extracts: Total extracts from C-6 glioma cells prepared with anisomycin treatment to serve as a positive control. Supplied in SDS Sample Buffer.

非磷酸化的p38 MAPK对照细胞提取物:未用茴香霉素处理的C-6 glioma细胞总提取物,作为阴性对照,加入到SDS样品缓冲液。磷酸化的p38 MAPK对照细胞提取物:用茴香霉素处理的C-6 glioma细胞总提取物,作为阳性对照,加入到SDS样品缓冲液。

Western blot analysis of p38 MAPK Control Cell extracts using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb

#4511 (upper) and p38 MAPK Antibody #9212 (lower).

Applications

Western Blots: For controls, CST recommends using 15 µl of phosphorylated and nonphosphorylated p38 MAP Kinase Control Cell Extracts. Boil for 2 minutes prior to use.

Western Blots:作为对照,CST建使用15 µl磷酸化和非磷酸化的p38 MAP Kinase Control Cell Extracts,在使用之前煮沸2 min。

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). 
 SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

p38 MAP kinase (MAPK),也被称为RK (1)或CSBP (2),是酵母中HOG激酶在哺乳动物中的同源蛋白,在细胞因子和应急诱导的信号转导过程中起重要作用。(1-4)。已经发现了4种亚型的p38 MAPK,分别是p38α, β, γ(也被称为Erk6或 SAPK3)以及δ (也被称为 SAPK4)。与SAPK/JNK通路类似,p38 MAPK可以被多种细胞压力所激活,包括渗透压休克,炎症因子,脂多糖(LPS),紫外线照射和生长因子(1-5)。MKK3, MKK6和SEK能够使p38 MAPK的Thr180 和Tyr182位点磷酸化,从而激活p38 MAPK。激活的p38 MAPK能够磷酸化并激活MAPKAP kinase 2 (3),进而最终磷酸化ATF-2 (5), Max (6)和MEF2等转录因子(5-8)。SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole)是p38 MAPK的选择性抑制剂。该复合物通过抑制p38 MAPK及后续HSP27的磷酸化,抑制MAPKAPK-2的激活(9)。SB203580能够结合到p38 MAPK与ATP结合的位置从而抑制p38 MAPK的催化活性,但不会通过上游激酶抑制p38 MAPK的磷酸化(10)。

  1. Rouse, J. et al. (1994) Cell 78, 1027-37.
  2. Han, J. et al. (1994) Science 265, 808-11.
  3. Lee, J.C. et al. (1994) Nature 372, 739-46.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-49.
  5. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
  6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
  7. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.
  9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
  10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

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