Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211

CSBP   HOGK   p38   p38-MAP-kinase   p38-MAPK   p38-β   p38-γ   p38-δ   p38β   p38γ   p38δ   RK   SAPK-2  

No. Size Price
9211L 600 µl ( 60 western blots ) ¥8,792.00 现货查询 购买询价
9211S 200 µl ( 20 western blots ) ¥3,780.00 现货查询 购买询价
9211 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster,Pig,S. cerevisiae, Endogenous 43 Rabbit
IP 1:50
F 1:200
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Hamster, Zebrafish, Bovine,

Specificity / Sensitivity

Phospho-p38 MAPK (Thr180/Tyr182) Antibody detects endogenous levels of p38 MAPK only when activated by phosphorylation at threonine 180 and tyrosine 182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182.

Phospho-p38 MAPK (Thr180/Tyr182) Antibody兔多抗能够检测内源性Thr180位点和Tyr182位点磷酸化的p38 MAPK激酶水平。该抗体不能与p42/44 MAPK和SAPK/JNK的磷酸化形式发生交叉反应。该抗体也能与Thr180位点、Tyr182位点单独磷酸化的p38 发生反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAPK. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源p38 MAPK蛋白Thr180/Tyr182位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from UV-treated NIH/3T3 cells, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or control p38 MAPK Antibody #9212 (lower).western blot方法检测细胞提取物:UV处理的NIH/3T3细胞,使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) Antibody (上图) 和control p38 MAPK Antibody #9212 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) Antibody compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物:未经处理(蓝色)和茴香霉素处理的(绿色)Jurkat细胞,使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) Antibody,红色曲线表示非特异性阴性对照抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or p38 MAPK Antibody #9212 (lower).western blot方法检测细胞提取物:未处理和茴香霉素处理的C6细胞、未处理和UV处理的NIH/3T3细胞,使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) Antibody (上图) 和p38 MAPK Antibody #9212 (下图)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with Phospho-p38 MAPK (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).激光共聚焦荧光法检测HeLa细胞,左侧未经处理的样品无染色,右侧经紫外照射的样品显示出细胞核的位置。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记,呈红色。

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). 
 SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

p38 MAP kinase (MAPK),也被称为RK (1)或CSBP (2),是酵母中HOG激酶在哺乳动物中的同源蛋白,在细胞因子和应急诱导的信号转导过程中起重要作用。(1-4)。已经发现了4种亚型的p38 MAPK,分别是p38α, β, γ(也被称为Erk6或 SAPK3)以及δ (也被称为 SAPK4)。与SAPK/JNK通路类似,p38 MAPK可以被多种细胞压力所激活,包括渗透压休克,炎症因子,脂多糖(LPS),紫外线照射和生长因子(1-5)。MKK3, MKK6和SEK能够使p38 MAPK的Thr180 和Tyr182位点磷酸化,从而激活p38 MAPK。激活的p38 MAPK能够磷酸化并激活MAPKAP kinase 2 (3),进而最终磷酸化ATF-2 (5), Max (6)和MEF2等转录因子(5-8)。SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole)是p38 MAPK的选择性抑制剂。该复合物通过抑制p38 MAPK及后续HSP27的磷酸化,抑制MAPKAPK-2的激活(9)。SB203580能够结合到p38 MAPK与ATP结合的位置从而抑制p38 MAPK的催化活性,但不会通过上游激酶抑制p38 MAPK的磷酸化(10)。

  1. Rouse, J. et al. (1994) Cell 78, 1027-37.
  2. Han, J. et al. (1994) Science 265, 808-11.
  3. Lee, J.C. et al. (1994) Nature 372, 739-46.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-49.
  5. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
  6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
  7. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.
  9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
  10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

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