Cell Signaling Technology

Product Pathways - Innate Immunity

TRIM21 (D1O1D) Rabbit mAb #92043

No. Size Price
92043S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
92043 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 50 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TRIM21 (D1O1D) Rabbit mAb recognizes endogenous levels of total TRIM21 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val309 of human TRIM21 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, 16 hr; +), using TRIM21 (D1O1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human TRIM21 (hTRIM21; +), using TRIM21 (D1O1D) Rabbit mAb.

Background

Tripartite motif-containing protein 21 (TRIM21) is a cytosolic antiviral protein that is induced by interferons and acts as a cytosolic IgG receptor (1,2).  TRIM21 is an activator of the innate immune system and targets antibody bound virions to the proteasome via its E3 ubiquitin ligase activity, leading to degradation of the virions in the cytosol (3,4). Interestingly, TRIM21 has also been shown to negatively regulate pathogen-induced interferon production through the polyubiquitin-mediated degradation of multiple targets including IRF3, IRF7, and DDX41 (5,6,7).

  1. Keeble, A.H. et al. (2008) Proc Natl Acad Sci U S A 105, 6045-50.
  2. Sjöstrand, M. et al. (2013) J Immunol 191, 3753-63.
  3. McEwan, W.A. et al. (2013) Nat Immunol 14, 327-36.
  4. Mallery, D.L. et al. (2010) Proc Natl Acad Sci U S A 107, 19985-90.
  5. Higgs, R. et al. (2008) J Immunol 181, 1780-6.
  6. Young, J.A. et al. (2011) J Biol Chem 286, 6521-31.
  7. Zhang, Z. et al. (2013) Nat Immunol 14, 172-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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