Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody #9204

p70-S6K   p70s6   pstat3   S6K   S6Kinase  

No. Size Price
9204L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9204S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9204 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70, 85 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421/Ser424. This antibody also detects p85 S6 kinase when phosphorylated at the corresponding sites (Thr444/Ser447).

Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody可以检测Thr421/Ser424磷酸化的内源性p70 S6激酶。这些抗体也可以检测相对应位点(Thr444/Ser447)磷酸化的p85 S6激酶。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr421/Ser424 of human p70 S6 kinase. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用人p70 S6激酶Thr421/Ser424周围残基相对应的合成磷酸肽免疫动物生产的,抗体采用蛋白A和肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated, serum-treated or EGF-treated (100 ng/ml), using Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody (upper) or p70 S6 Kinase Antibody #9202 (lower).

Western blot分析293细胞提取物,分组为未处理组,血清处理组和EGF处理组(100 ng/ml)。所用抗体为Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody (上) 或p70 S6 Kinase Antibody #9202 (下)。



Immunoprecipitation of phosphorylated p70 S6 Kinase from 293 cell extracts (cells were serum-stimulated as indicated) under nondenaturing or denaturing conditions, followed by Western blot analysis, using Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody.

Western blot 后,免疫沉淀分析非还原和还原条件下,血清处理后的293细胞p70 S6激酶磷酸化,所用抗体为Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody。


p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal (1). Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (3). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (4,5). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,6,7). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase (8).

p70 S6激酶是有丝分裂原活化的丝氨酸/苏氨酸蛋白激酶,是细胞生长和G1期细胞周期正常进行必需的(1,2)。P70 S6激酶可以磷酸化核糖体40S亚基的S6蛋白并参与5‘ oligopyrimidine tract mRNAs的翻译控制(1)。另一个亚型,P85 S6激酶,和p70 S6激酶由相同的基因转录,但氨基端有23个额外的残基,编码核定位信号(1)。两种亚型都处于有丝分裂原激活的PI-3K和rapamycin靶点FRAP/mTOR的信号通路下游,这条通路独立于Ras /MAP激酶级联反应(1)。p70 S6激酶活性取决于在催化、连接和伪底物结构域的多种磷酸化修饰(1)。催化结构域的Thr229和连接结构域的Thr389是调节激酶功能最关键的磷酸化修饰位点(1)。而且与P70激酶体内活性最相关的磷酸化修饰位点是Thr389(3)。Thr389磷酸化可以激活PDK1的Thr229磷酸化(4,5)。这个位点的磷酸化受到各种生长因子,如胰岛素,表皮生长因子和成纤维细胞生长因子,以及血清和一些G蛋白偶联受体配体,wortmannin,LY294002(PI-3K抑制剂)和rapamycin(FRAP/mTOR抑制剂)的刺激(1,6,7)。Ser411,Thr421和Thr424位于伪底物结构域的丝氨酸脯氨酸丰富区(1)。这些位点的磷酸化被认为是通过解除伪底物的抑制进而激活p70 S6激酶(1,2)。另一个对LY294002和rapamycin敏感的磷酸化位点,Ser371,是mTOR的体的底物,与某种抗rapamycin的P70 S6激酶突变体有关(8)。

  1. Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82.
  2. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  3. Weng, Q.P. et al. (1998) J Biol Chem 273, 16621-9.
  4. Pullen, N. et al. (1998) Science 279, 707-10.
  5. Alessi, D.R. et al. (1998) Curr Biol 8, 69-81.
  6. Polakiewicz, R.D. et al. (1998) J Biol Chem 273, 23534-41.
  7. Fingar, D.C. et al. (2002) Genes Dev 16, 1472-87.
  8. Saitoh, M. et al. (2002) J Biol Chem 277, 20104-12.

Application References

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