Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198

CREB1   pCREB   pCREB1   sc-101663   sc-7978  

No. Size Price
9198L 300 µl ( 30 western blots ) ¥9,325.00 现货查询 购买询价
9198S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
9198 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 43 Rabbit IgG
IHC-P 1:800
F 1:800
IF-F 1:800
IF-IC 1:800
IHC-F 1:400
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Zebrafish,

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗可识别内源性的Ser133磷酸化的CREB。该抗体也可识别磷酸化的CREB相关蛋白ATF-1。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.

该单克隆抗体由合成的人类CREB蛋白Ser133位点附近磷酸化肽段免疫动物而制成。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SK-N-MC cells, untreated (blue) or IBMX- and forskolin-treated (green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb compared to a nonspecific negative control antibody (red).

流式细胞仪分析未处理(蓝色)或用IBMX-和forskolin处理(绿色)的SK-N-MC细胞,使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗和非特异性阴性对照抗体(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and FGF-treated, using Phospho-CREB (Ser133) (87G3) Rabbit mAb (upper) or CREB (48H2) Rabbit mAb #9197 (lower).

Western blot分析SK-N-MC细胞(未处理或Forskolin和FGF处理),使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗(上图)或CREB (48H2) Rabbit mAb 兔单抗#9197(下图)。

IF-IC

IF-IC

Confocal microscopic images of SK-N-MC cells showing nuclear stain after 25 minute treatment with Forskolin and IBMX using Phospho-CREB (Ser133) (87G3) Rabbit mAb (left, red) compared to untreated cells (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

对SK-N-MC细胞进行25分钟Forskolin和IBMX处理后,共聚焦显微图像显示出核染色,使用Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗(左图,红色),与未处理对照组比对(右图)。蓝色伪彩=DRAQ5 ® #4084(荧光DNA染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb.

免疫组化分析石蜡包埋的人肺癌,使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-CREB (Ser133) (87G3) Rabbit mAb in the presence of control peptide (left) or Phospho-CREB (Ser133) Blocking Peptide #1090 (right).

免疫组化分析石蜡包埋的人乳腺癌,使用的抗体是Phospho-CREB (Ser133)(87G3) Rabbit mAb 兔单抗,其中左图为存在对照肽,右图为存在Phospho-CREB (Ser133)Blocking Peptide 封闭肽 #1090 。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) (87G3) Rabbit mAb.

免疫组化分析石蜡包埋的人肾细胞癌,未处理(左)或λ磷酸酶处理(右),使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SK-N-MC cells, untreated (left) or IBMX- and forskolin-treated (right), showing induced nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb.

免疫组化分析石蜡包埋的SK-N-MC细胞,未处理(左)或IBMX和Forskolin处理(右),显示出诱导的核染色,使用的抗体是 Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗。

IF-F

IF-F

Conofocal immunofluorescent images of rat dentate gyrus, either sham-operated (left) or 15 min ischemia followed by 30 min (center) and 4 h (right) reperfusion, labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb (red), Neurofilament-L (DA2) Mouse mAb #2835 (blue) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.

共聚焦免疫荧光分析大鼠齿状回,假手术组(左图)或15分钟缺血后再灌注30分钟(中图)和4小时(右图),使用Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗(红色)、Neurofilament-L (DA2) Mouse mAb 鼠单抗#2835(蓝色)和 Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854进行标记。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using Phospho-CREB (Ser133)(87G3) Rabbit mAb.

免疫组化分析冰冻H1650 异种移植物,显示出核定位,使用的抗体是Phospho-CREB (Ser133)(87G3) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using Phospho-CREB (Ser133) (87G3) Rabbit mAb.

免疫组化分析石蜡包埋的人肺癌,使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30 μM) for 1h and either 20 μl of Phospho-CREB (Ser133) (87G3) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒,将Forskolin #3828 (30 uM)处理1小时后的4 x 10^6 293细胞的染色质,与20 μl Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人ALS2 外显子1的 primers,SimpleChIP® Human NR4A3 Promoter Primers #4829,和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR用来定量富集的DNA。每个样本中被沉淀的DNA量用总投入量(等于1)的百分比表示。

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

CREB的是一个bZIP转录因子,通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号,调节很多的细胞反应。CREB的在许多组织中表达,它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用(1-3)。此外,CREB信号在数种生物体中涉及学习和记忆(4-6)。CREB能够通过与不同二聚体相互作用,选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活,激活可由多种信号通路完成,包括ERK,Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK,MSK,CaMKIV, MAPKAPK-2(7-9)。

  1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
  2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
  3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
  4. Dash, P.K. et al. (1990) Nature 345, 718-21.
  5. Yin, J.C. et al. (1994) Cell 79, 49-58.
  6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
  7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
  8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
  9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.
  10. Devi, L. and Ohno, M. (2015) Transl Psychiatry 5, e562.

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