Cell Signaling Technology

Product Pathways - Neuroscience

CREB (48H2) Rabbit mAb #9197

sc-58  

No. Size Price
9197L 300 µl ( 30 western blots ) ¥8,023.00 现货查询 购买询价
9197S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9197 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 43 Rabbit IgG
IP 1:250
IHC-P 1:6400
F 1:400
IF-F 1:800
IF-IC 1:800
IHC-F 1:6400
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen), ChIP=Chromatin IP,

Specificity / Sensitivity

CREB (48H2) Rabbit mAb detects endogenous levels of total CREB protein.

CREB (48H2) Rabbit mAb 兔单抗识别内源性的总CREB蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CREB protein.

该单克隆抗体由人类 CREB蛋白氨基末端附近的氨基酸序列对应的重组蛋白免疫动物而制成。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using CREB (48H2) Rabbit mAb.

免疫组化分析石蜡包埋的人肺癌,使用的抗体是CREB (48H2) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using CREB (48H2) Rabbit mAb.

免疫组化分析石蜡包埋的人非霍奇金淋巴瘤,使用的抗体是CREB (48H2) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human astrocytoma, using CREB (48H2) Rabbit mAb.

免疫组化分析石蜡包埋的人胶质细胞瘤,使用的抗体是CREB (48H2) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain, using CREB (48H2) Rabbit mAb.

免疫组化分析石蜡包埋的小鼠大脑,使用的抗体是CREB (48H2) Rabbit mAb 兔单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-N-MC cells showing nuclear stain with CREB (48H2) Rabbit mAb (A, red) compared to an isotype control (B). Blue pseudocolor =DRAQ5®(fluorescent DNA dye).

共聚焦免疫荧光分析SK-N-MC细胞,显示出核染色,使用CREB (48H2) Rabbit mAb 兔单抗(A图,红色),与同型对照组比对(B图)。蓝色伪彩=DRAQ5® (荧光DNA染料)。

IF-F

IF-F

Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析小鼠小脑,使用CREB (48H2) Rabbit mAb 兔单抗(红色)和Neurofilament-L (DA2) Mouse mAb 鼠单抗#2835(绿色)进行标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SK-N-MC cells using CREB (48H2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

流式细胞仪分析SK-N-MC细胞,使用CREB (48H2) Rabbit mAb 兔单抗(蓝)和非特异的阴性对照抗体(红)。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb.

免疫组化分析冰冻H1650异植物,使用的抗体是CREB (48H2) Rabbit mAb 兔单抗,显示出核定位。

Western Blotting

Western Blotting

Western Blot analysis of extracts from SK-N-MC, COS, NIH/3T3, C6 and Drosophila S2 cells, using CREB (48H2) Rabbit mAb.

Western blot分析SK-N-MC、COS、NIH/3T3、C6和Drosophila S2细胞提取物,使用的抗体是CREB (48H2) Rabbit mAb 兔单抗。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells, treated with Forskolin #3828 (30 μM) for 1h and either 10 μl of CREB (48H2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒,将Forskolin #3828 (30 μM)处理1小时后的4 x 10^6 293细胞的染色质,与10 μl CREB (48H2) Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人ALS2 外显子1的primers、 SimpleChIP® Human NR4A3 Promoter Primers #4829, 和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR用来定量富集的DNA。每个样本中被沉淀的DNA量用总投入量(等于1)的百分比表示。

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

CREB的是一个bZIP转录因子,通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号,调节很多的细胞反应。CREB的在许多组织中表达,它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用(1-3)。此外,CREB信号在数种生物体中涉及学习和记忆(4-6)。CREB能够通过与不同二聚体相互作用,选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活,激活可由多种信号通路完成,包括ERK,Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK,MSK,CaMKIV, MAPKAPK-2(7-9)。

  1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
  2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
  3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
  4. Dash, P.K. et al. (1990) Nature 345, 718-21.
  5. Yin, J.C. et al. (1994) Cell 79, 49-58.
  6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
  7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
  8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
  9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.
  10. Devi, L. and Ohno, M. (2015) Transl Psychiatry 5, e562.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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