Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-CREB (Ser133) (1B6) Mouse mAb #9196

CREB1   pCREB   pCREB1  

No. Size Price
9196L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9196S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9196 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 43 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-CREB (Ser133) (1B6) Mouse mAb detects endogenous levels of CREB only when phosphorylated at serine 133. This antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Phospho-CREB (Ser133) (1B6)Mouse mAb 鼠单抗可识别内源性的Ser133磷酸化的CREB。该抗体也可识别磷酸化的CREB相关蛋白ATF-1。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.

该单克隆抗体由合成的人类CREB蛋白Ser133位点附近磷酸化肽段免疫动物而制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC cells, untreated or treated with forskolin and FGF(lanes 1 and 2) or 293 cells. untreated or UV treated (lanes 3 and 4), using Phospho-CREB (Ser133) (1B6) Mouse mAb.

Western blot分析未处理或Forskolin和FGF处理的SK-N-MC细胞(列1和列2)或未处理或UV处理的293细胞 (列3或列4),使用的抗体是Phospho-CREB (Ser133) (1B6) Mouse mAb 鼠单抗。

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

CREB的是一个bZIP转录因子,通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号,调节很多的细胞反应。CREB的在许多组织中表达,它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用(1-3)。此外,CREB信号在数种生物体中涉及学习和记忆(4-6)。CREB能够通过与不同二聚体相互作用,选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活,激活可由多种信号通路完成,包括ERK,Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK,MSK,CaMKIV, MAPKAPK-2(7-9)。

  1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
  2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
  3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
  4. Dash, P.K. et al. (1990) Nature 345, 718-21.
  5. Yin, J.C. et al. (1994) Cell 79, 49-58.
  6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
  7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
  8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
  9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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