Cell Signaling Technology

Product Pathways - MAPK Signaling

p44/42 MAPK (Erk1/2) Control Cell Extracts #9194

control lysate   erk   erk1   Erk1/2   erk2   jurkat   map kinase   mapk   negative control   p42   p44   p44/42   phorbol ester   pma   positive control   tpa   u0126  

No. Size Price
9194S 150 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9194 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

p44/42 MAPK (Erk1/2) Negative Control Cell Extracts: Total cell extracts from Jurkat cells treated with U0126 (MEK1/2 Inhibitor) #9903 at 10 μM for 1 hour, to serve as a negative control. Supplied in SDS Sample Buffer. 
 p44/42 MAPK (Erk1/2) Positive Control Cell Extracts: Total cell extracts from Jurkat cells treated with TPA #9905 at 200 nM for 10 minutes, to serve as a positive control. Supplied in SDS Sample Buffer.

p44/42 MAPK (Erk1/2) Negative Control Cell Extracts:U0126 (MEK1/2 Inhibitor) #9903 10 μM 1 h处理的Jurkat细胞总提取物作为阴性对照,加入SDS样品缓冲液。p44/42 MAPK (Erk1/2) Positive Control Cell Extracts:TPA #9905 200 nM 10 min处理的Jurkat 细胞总提取物作为阳性对照,加入SDS样品缓冲液。

Western Blotting

Western Blotting

Western blot analysis of p44/42 MAPK (Erk1/2) Control Cell Extracts, treated with either U0126 or TPA, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 (left) or p44/42 MAPK (Erk1/2) Antibody #9102 (right).Western blot方法检测p44/42 MAPK (Erk1/2) Control Cell Extracts:用U0126 、TPA处理细胞。使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 (左侧) 和p44/42 MAPK (Erk1/2) Antibody #9102 (r右侧)。

Western Blotting

Western Blotting

Western blot analysis of p44/42 MAPK (Erk1/2) Control Cell Extracts, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 (green) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (red).Western blot方法检测p44/42 MAPK (Erk1/2) Control Cell Extracts,使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 (绿色)和p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (红色)。

Applications

Western Blots: As controls, CST recommends using 15 μl of U0126- and TPA-treated p44/42 MAPK (Erk1/2) Cell Extracts. Boil for 3-5 minutes before use.

Western Blots分析:我们建议用U0126、TPA处理的 p44/42 MAPK (Erk1/2) Cell Extracts作为对照。在使用之前煮沸3-5 min。

Background

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

丝裂原活化蛋白激酶(MAPKs)是一个具有广泛保守性的丝氨酸/苏氨酸蛋白激酶家族,在许多细胞过程中起作用,比如细胞增殖、分化、运动和死亡。p44/42 MAPK (Erk1/2)信号转导通路能在应答各种胞外刺激后被激活,这些刺激因子包括有丝分裂原、生长因子和细胞因子(1-3),并且被研究者认为是诊断和治疗癌症的重要靶点(4)。受到刺激后,三个不同层次的蛋白激酶级联反应就连续被开启,这三个层次的分子包含MAPKKK(MAP3K)、MAPKK(MAP2K)、MAPK。多种p44/42 MAP3Ks已经被鉴定出来,包括Raf家族成员,以及Mos、Tpl2/Cot。MEK1、MEK2是该通路中主要的MAPKKs(5,6)。MEK1、MEK2通过分别磷酸化p44和p42蛋白的活化环内的Thr202/Tyr204位点和Thr185位点/Tyr187位点,从而激活p44、p42。目前已经鉴定出p44/42的一些下游靶点,包括p90RSK (7) 和下游转录因子Elk-1 (8,9)。双特异性(Thr/Tyr) MAPK磷酸酶家族成员DUSPs、MKPs (10),以及MEK抑制子如U0126、 PD98059,可以负调节p44/42。

  1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
  2. Baccarini, M. (2005) FEBS Lett 579, 3271-7.
  3. Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
  4. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
  5. Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
  6. Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
  7. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  8. Marais, R. et al. (1993) Cell 73, 381-93.
  9. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
  10. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.

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