Cell Signaling Technology

Product Pathways - Neuroscience

CREB Control Cell Extracts #9193

CREB  

No. Size Price
9193S 100 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9193 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated CREB Cell Extracts: Total cell extracts from SK-N-MC cells prepared without treatment serve as a negative control. Supplied in SDS Sample Buffer. 
Phosphorylated CREB Cell Extracts: Total cell extracts from SK-N-MC cells prepared with IBMX and forskolin treatment serve as a positive control. Supplied in SDS Sample Buffer.

非磷酸化CREB的细胞提取物:未经任何处理的SK-N-MC全细胞提取物作为阴性对照。以SDS样本缓冲液提供。磷酸化CREB的细胞提取物:经IBMX和forskolin处理的SK-N-MC全细胞提取物作为阳性对照。以SDS样本缓冲液提供。

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and IBMX-treated, using 4276 Phospho-CREB (Ser133) (D1G6) Rabbit mAb (upper) or 4820 CREB (D76D11) Rabbit mAb (lower).

Western blot 分析未经处理或forskolin和IBMX 处理的SK-N-MC 细胞提取物,使用的抗体是#4276 Phospho-CREB (Ser133) (D1G6) Rabbit mAb 兔单抗 (上图) 或 #4820 CREB (D76D11) Rabbit mAb 兔单抗 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC cells, untreated (-) or treated with Forskolin #3828 (30 μM) and IBMX (0.5 mM) for 30 min (+), using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (upper) and CREB (48H2) Rabbit mAb #9197 (lower).

Western blot 分析未经处理或Forskolin #3828 (30 μM) 和 IBMX (0.5 mM) 处理30分钟的SK-N-MC 细胞提取物,使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗#9198 (上图)和 CREB (48H2) Rabbit mAb 兔单抗 #9197 (下图)。

Applications

As controls, we recommend using 10 µl of phosphorylated and nonphosphorylated CREB control extracts. Boil sample before use.

作为对照,我们推荐使用10μl的磷酸化和非磷酸化CREB的提取物。使用前煮沸样本。

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

CREB的是一个bZIP转录因子,通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号,调节很多的细胞反应。CREB的在许多组织中表达,它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用(1-3)。此外,CREB信号在数种生物体中涉及学习和记忆(4-6)。CREB能够通过与不同二聚体相互作用,选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活,激活可由多种信号通路完成,包括ERK,Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK,MSK,CaMKIV, MAPKAPK-2(7-9)。

  1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
  2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
  3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
  4. Dash, P.K. et al. (1990) Nature 345, 718-21.
  5. Yin, J.C. et al. (1994) Cell 79, 49-58.
  6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
  7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
  8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
  9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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