Cell Signaling Technology

Product Pathways - MAPK Signaling

Elk-1 Control Proteins #9183

ELK  

No. Size Price
9183S 150 µl ( 10 western blots ) ¥1,413.00 现货查询 购买询价
9183 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated Elk1 fusion protein: Bacterially expressed Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC. 
 Phosphorylated Elk1 fusion protein: Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.

非磷酸化的Elk1融合蛋白:细菌中表达的Elk1融合蛋白,作为阴性对照来使用。以SDS样品缓冲液的形式供应,-20ºC保存。磷酸化的Elk1融合蛋白:Erk2酶磷酸化的Elk1蛋白,作为阳性对照使用。以SDS样品缓冲液的形式供应,-20ºC保存。

Western Blotting

Western Blotting

Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody #9186 (upper) or control Elk-1 Antibody #9182 (lower).

Directions for Use

As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.

对于对照组,我们建议用15 µl磷酸化和非磷酸化的Elk-1蛋白对照提取物。Loading之前先煮沸样品。

Background

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).

转录因子Elk-1是一个三元复合物的组成部分,该复合物能结合血清效应因子(SRE),调节基因活性以应答血清和生长因子(1-3)。MAP激酶能将Elk-1蛋白羧端的一串S/T基序磷酸化,这些位点的磷酸化,特别是丝氨酸(383位点)的磷酸化,对Elk-1蛋白的转录活性很重要。Elk-1蛋白似乎是活化的MAP激酶直接的靶点:(a)生化研究表明Elk-1蛋白是MAP激酶很好的底物;(b) Elk-1蛋白磷酸化和活化的动力学与MAP激酶活性有关;(c) MAP激酶的干扰突变体会阻碍体内Elk-1蛋白的活化。其他研究表明丝氨酸(383位点)磷酸化的Elk-1蛋白也是应激性活化激酶SAPK/JNK的靶点(4,5).。

Western Blots: For controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.

对于对照组,我们建议用15 µl磷酸化和非磷酸化的Elk-1蛋白对照提取物。Loading之前先煮沸样品。

  1. Marais, R. et al. (1993) Cell 73, 381-393.
  2. Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824.
  3. Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
  4. Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.
  5. Whitmarsh, A.J. et al. (1995) Science 269, 403-407.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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