Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat1 (Ser727) Antibody #9177


No. Size Price
9177S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9177 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 91 Rabbit
IF-IC 1:100
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Monkey, Bovine,

Specificity / Sensitivity

Phospho-Stat1 (Ser727) Antibody detects endogenous levels of Stat1α only when phosphorylated at Ser727. This site is deleted in Stat1β. This antibody does not significantly cross-react with the corresponding phosphorylated residues of other Stat proteins.

Phospho-Stat1 (Ser727) Antibody抗体只能检测内源的在 Ser727 位点磷酸的Stat1α蛋白。这个位点在Stat1β中已删除。此抗体不与对应的磷酸化残基的其它Stat 蛋白反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser727 of human Stat1. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆是通过合成人源对应的Stat1 Ser727位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 (human), NIH/3T3 (mouse), and C6 (rat) cells, untreated or UV-stimulated, using Phospho-Stat1 (Ser727) Antibody



Confocal immunofluorescent analysis of HeLa cells, untreated (left), IFN-γ treated (center) or IFN-γ and phosphatase treated (right), labeled with Phospho-Stat1 (Ser727) Antibody (green) and Pan-Keratin (C11) Mouse mAb #4545 (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Hela cells, phosphatase-treated (red), untreated (blue) or UV-treated (green), using Phospho-Stat1 (Ser727) Antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 20 μl of Phospho-Stat1 (Ser727) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Stat1转录因子的激活是通过一系列的配体完成 (1) 这对IFN-α和 IFN-γ的响应非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1的形成二聚体, 入核和DNA结合(4)。 Stat1 蛋白是以成对的形式存在Stat1α (91 kDa) 和 另外的剪切方式Stat1β (84 kDa)。在很多细胞中,这两种形式都是通过IFN-α激活。但是有时 Stat1α是被 IFN-γ激活。 Stat1这种不适当的激活方式在存在于很多肿瘤中(5)。除了酪氨酸磷酸化,Stat1 也能通过p38有丝分裂激活蛋白激酶依赖的通路在Ser727位点磷酸化而激活,从而响应IFN-α 和其它的细胞胁迫(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

  1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
  2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
  3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
  4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
  5. Frank, D.A. (1999) Mol Med 5, 432-56.
  6. Wen, Z. et al. (1995) Cell 82, 241-50.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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