Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat1 Control Cell Extracts #9173

signal transducer and activator of transcription  

No. Size Price
9173S 100 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9173 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated Stat1 Control Cell Extracts: Total cell extracts from HeLa cells prepared without treatment serve as a negative control. Supplied in SDS Sample Buffer. 
 Phosphorylated Stat1 Control Cell Extracts: Total cell extracts from HeLa cells prepared with 100 ng/ml interferon-a 5 minute treatment serve as a positive control. Supplied in SDS Sample Buffer.

未磷酸化Stat1对照细胞抽提液:未经处理的HeLa细胞的总抽提液作为阴性对照。保存在SDS样品缓冲液中。磷酸化Stat1对照细胞抽提液:经过100 ng/ml 干扰素-a 处理5 min 的HeLa总细胞抽提液作为阳性对照。抽提液保存在SDS上样缓冲液中。

Applications

As controls, we recommend using 10 µl of phosphorylated and nonphosphorylated Stat1 control cell extractsThese lysates are useful for Phospho-Stat1 (Tyr701) Antibody #9171. However, they are not useful for Phospho-Stat1 (Ser727) Antibody #9177.

作为对照,我们推荐用10 µl 磷酸化和未磷酸化Stat1对照细胞抽提液。这些抽提液对Phospho-Stat1 (Tyr701) Antibody #9171有效,但是对Phospho-Stat1 (Ser727) Antibody #9177无效。

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Stat1 转录因子的激活是通过一系列的配体完成 (1) 这对IFN-α和 IFN-γ的响应非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1的形成二聚体, 入核和DNA结合(4)。 Stat1 蛋白是以成对的形式存在Stat1α (91 kDa) 和 另外的剪切方式Stat1β (84 kDa)。在很多细胞中,这两种形式都是通过IFN-α激活。但是有时 Stat1α是被 IFN-γ激活。 Stat1这种不适当的激活方式在存在于很多肿瘤中(5)。除了酪氨酸磷酸化,Stat1 也能通过p38有丝分裂激活蛋白激酶依赖的通路在Ser727位点磷酸化而激活,从而响应IFN-α 和其它的细胞胁迫(6)。丝氨酸磷酸化对最大化诱导Stat1介导的基因激活也是必需的。

  1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
  2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
  3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
  4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
  5. Frank, D.A. (1999) Mol Med 5, 432-56.
  6. Wen, Z. et al. (1995) Cell 82, 241-50.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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