Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat1 Antibody #9172

1SGF-3 p91/p84   signal transducer and activator of transcription   stat1   Stat1-alpha/beta  

No. Size Price
9172L 300 µl ( 30 western blots ) ¥7,504.00 现货查询 购买询价
9172S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9172T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
9172 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 84, 91 Rabbit
IP 1:100
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog,

Specificity / Sensitivity

Stat1 Antibody detects endogenous levels of total Stat1 protein. The antibody detects both Stat1alpha (91kDa) and Stat1beta (84 kDa) isoforms.

Stat1 抗体能够检测内源Stat1蛋白总体水平。此抗体能检测Stat1alpha (91kDa) 和 Stat1beta (84 kDa)两种不同贵的剪切形式。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Stat1. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成对应人源Stat1序列的多肽来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-MEL-28 cells, untreated or IFN-alpha-treated (100 ng/ml), using Phospho-Stat1 (Tyr701) Antibody #9171 (upper) or Stat1 Antibody (lower).Western免疫印迹。用Phospho-Stat1 (Tyr701) Antibody #9171 (上图) or Stat1 Antibody (下图)研究未经处理的或经 IFN-alpha(100 ng/ml)处理的SK-MEL-28 细胞的细胞提取液。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with non-targeted (control) siRNA or transfection with Stat1 siRNA. Stat1 was detected using Stat1 Antibody #9172, and p42 was detected using p42 MAPK Antibody #9108. The Stat1 Antibody confirms silencing of Stat1 expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity.Western免疫印迹。HeLa细胞经过对照转染,转染了non-targeted (对照) siRNA ,转染了Stat1 siRNA的质粒48小时后抽提细胞液,用Stat1 Antibody #9172研究Stat1蛋白, 用p42 MAPK Antibody #9108研究 p42 蛋白。Stat1 抗体是为了验证基因沉默靶基因Stat1蛋白的表达, p42 MAPK 抗体是为了蛋白上样的参照和siRNA 的特异性。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 10 μl of Stat1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。HT-1080 细胞培养至4 x 106 , 并经IFN-γ (50 ng/ml,30 min) 处理,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用10 μl of Stat1 抗体或2 μl Normal Rabbit IgG #2729 抗体。用human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148 和SimpleChIP® Human α Satellite Repeat Primers #4486. 对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Stat1 转录因子的激活是通过一系列的配体完成 (1) 这对IFN-α和 IFN-γ的响应非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1的形成二聚体, 入核和DNA结合(4)。 Stat1 蛋白是以成对的形式存在Stat1α (91 kDa) 和 另外的剪切方式Stat1β (84 kDa)。在很多细胞中,这两种形式都是通过IFN-α激活。但是有时 Stat1α是被 IFN-γ激活。 Stat1这种不适当的激活方式在存在于很多肿瘤中(5)。除了酪氨酸磷酸化,Stat1 也能通过p38有丝分裂激活蛋白激酶依赖的通路在Ser727位点磷酸化而激活,从而响应IFN-α 和其它的细胞胁迫(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

  1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
  2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
  3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
  4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
  5. Frank, D.A. (1999) Mol Med 5, 432-56.
  6. Wen, Z. et al. (1995) Cell 82, 241-50.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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