Cell Signaling Technology

Product Pathways - MAPK Signaling

c-Jun (60A8) Rabbit mAb #9165

AP-1   cJun   p39   rabbit monoclonal   sc-1694   sc-45  

No. Size Price
9165L 300 µl ( 30 western blots ) ¥7,504.00 现货查询 购买询价
9165S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9165T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
9165 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 43, 48 Rabbit IgG
IP 1:50
IHC-P 1:400
IF-IC 1:50
IHC-F 1:400
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen), ChIP=Chromatin IP,

Specificity / Sensitivity

c-Jun (60A8) Rabbit mAb detects endogenous levels of total c-Jun protein, regardless of phosphorylation state.

c-Jun (60A8) Rabbit mAb兔单抗能够检测内源性c-Jun总蛋白水平,无论是否被磷酸化。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-c-Jun protein corresponding to the amino-terminal sequence of human c-Jun.

该单克隆抗体是采用与人源c-Jun蛋白胺端序列相一致的合成GST-c-Jun蛋白免疫动物而产生的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人结肠癌组织,使用的抗体为c-Jun (60A8) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells, untreated or UV-treated, using c-Jun (60A8) Rabbit mAb.Western blot检测细胞提取物:未经处理和用紫外照射的NIH/3T3 和SK-N-MC 细胞,是用的抗体是c-Jun (60A8) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人卵巢癌组织,使用的抗体为c-Jun (60A8) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human astrocytoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人星形细胞瘤 组织,使用的抗体为c-Jun (60A8) Rabbit mAb。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的冷冻H1650移植瘤组织,显示出细胞核的位置。使用的抗体为c-Jun (60A8) Rabbit mAb。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, using c-Jun (60A8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞,使用的抗体是c-Jun (60A8) Rabbit mAb (绿色)。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记,呈红色。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of c-Jun (60A8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

c-Jun是Jun家族的一个成员,Jun家族成员包括c-Jun, JunB, 和 JunD。c-Jun是转录因子激活蛋白-1(AP-1)的一个组分。AP-1是由Fos和Jun形成的二聚体以及 ATF家族成员组成的,能够结合并激活TRE/AP-1元件的转录(1)。包括生长因子,细胞因子和压力在内的细胞外信号能够激活AP-1依赖的转录过程。C-Jun的转录活性是通过SAPK/JNK对其中的Ser63和Ser73的磷酸化来调控的(2)。小鼠敲除实验表明c-Jun对于胚胎形成是至关重要的(3),进一步的研究发现c-Jun在多种组织和发育过程中发挥作用,包括轴突再生(4),肝脏再生(5)和T细胞发育(6)。AP-1所调控的基因发挥了多种生物学功能,包括细胞增殖、分化、凋亡、转化,侵润和转移,这依赖于细胞种类和具体情况(7-9)。其他的AP-1所调控的靶基因还能够调节细胞存亡,以及低氧和血管生成(8,10)。研究提示c-Jun可以作为癌症、血管重构、急性炎症反应和风湿性关节炎的重要治疗靶点(11,12)。

  1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
  2. Davis, R.J. (2000) Cell 103, 239-52.
  3. Hilberg, F. et al. (1993) Nature 365, 179-81.
  4. Raivich, G. et al. (2004) Neuron 43, 57-67.
  5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
  6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
  7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
  9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
  10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
  11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
  12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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