Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Merlin (Ser518) Antibody #9163

neurofibromatosis   Neurofibromin 2   NF2   Schwannomerlin   Schwannomin  

No. Size Price
9163S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9163 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Merlin (Ser518) Antibody detects endogenous levels of merlin only when phosphorylated at Ser518.

Phospho-Merlin (Ser518) Antibody 兔多抗识别内源性的Ser518磷酸化的merlin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human merlin. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from PC3 cells, untreated or treated with λ phosphatase, using Phospho-Merlin (Ser518) Antibody (upper) or Merlin Antibody #9168 (lower).

Western blot分析PC3细胞(未处理或λ磷酸酶处理),使用的抗体是Phospho-Merlin (Ser518) Antibody 兔多抗(上图)或Merlin Antibody 兔多抗#9168(下图)。


Neurofibromatosis 2 (NF2) is an autosomal dominant inherited disorder that is characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors in the general population are caused by inactivation of the NF2 tumor suppressor gene. Merlin (moesin, ezrin, and radixin like protein), the NF2 gene product, has striking similarity to ezrin, radixin, and moesin (ERM). Regulation of merlin (aka schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4).

神经纤维瘤病2(NF2)是一种常染色体显性遗传病,以前庭神经鞘瘤,脑膜瘤和其他神经系统肿瘤的发生为特点。NF2家族性肿瘤患者和人群中散发的肿瘤,都是由NF2肿瘤抑制基因的失活引起。Merlin (moesin, ezrin和radixin样蛋白)都是NF2基因的产物,与ezrin, radixin和moesin (ERM)有惊人的相似性。merlin (aka schwannomin) 和 ERM蛋白的调控涉及家族成员中分子内部及分子间的头尾关系(1)。Merlin和ERM蛋白的功能是作为细胞膜和细胞骨架之间的连接,影响细胞的形态,极性和信号转导(2)。Rac/Cdc42的效应器p21激活的激酶(PAK)可以磷酸化Merlin的Ser518,负向调节Rac(3,4)。

  1. Ramesh, V. (2004) Nat. Rev. Neurosci. 5, 462-470.
  2. Bretscher, A. et al. (2002) Nat. Rev. Mol. Cell Biol. 3, 586-599.
  3. Xiao, G. H. et al. (2002) J. Biol. Chem. 277, 883-886.
  4. Kissil, J. L. et al. (2003) Mol. Cell 12, 841-849.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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