Cell Signaling Technology

Product Pathways - MAPK Signaling

MEK1/2 HeLa Control Cell Extracts #9160

lysates   MAPKK-1   MAPKK-2   MEK   MEK-1   MEK-2  

No. Size Price
9160S 200 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9160 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


Nonphosporylated MEK1/2 Cell Extracts: Total cell extracts from HeLa cells prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC. 
 Phosphorylated MEK1/2 Cell Extracts: Total cell extracts from HeLa cells prepared with TPA treatment (200 nM, 15 min) serve as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.

非磷酸化的MEK1/2 细胞提取物: 未经处理的HeLa细胞总提取物,可以作为阴性对照。细胞提取物以SDS样品缓冲液的形式提供。保存于-20ºC。磷酸化的MEK1/2细胞提取物:用TPA (200 nM, 15 min)处理的HeLa细胞总提取物,可以作为阳性对照。细胞提取物以SDS样品缓冲液的形式提供。保存于-20ºC。

Western Blotting

Western Blotting

Western blot analysis of extracts from Hela cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) RmAb #9154 (upper) or MEK1/2 (D1A5) Rabbit mAb #8727 (lower).


Western Blots: As controls, we recommend using 20 µl of phosphorylated and nonphosphorylated MEK1/2 control extracts. Boil sample before use.

Western Blots:作为对照,我们建议使用20 µl磷酸化和非磷酸化的MEK1/2对照提取物。在使用之前煮沸。


MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

MEK1、MEK2,也被称为MAPK或Erk激酶,它们是双特异性蛋白激酶,在丝裂原活化蛋白激酶级联反应中发挥作用,控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸(217 /221位点)的磷酸化而实现的,这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化,激活p44、 p42 MAPK激酶。

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
  4. Cowley, S. et al. (1994) Cell 77, 841-52.

Application References

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