Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-LATS1 (Ser909) Antibody #9157

Hippo  

No. Size Price
9157S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9157 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 140 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-LATS1 (Ser909) Antibody detects endogenous levels of total LATS1 protein only when phosphorylated on Ser909. Phospho-LATS1 (Ser909) Antibody能够检测内源性丝氨酸(909位)磷酸化的LATS1总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids surrounding Ser909 of human LATS1. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对LATS1蛋白丝氨酸(909位)的磷酸化肽段免疫动物,利用蛋白A和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with TPA, using Phospho-LATS1 (Ser909) Antibody. western blot方法检测未处理或TPA处理的HeLa细胞提取物,使用的抗体为Phospho-LATS1 (Ser909) Antibody。

Background

LATS1 (Large tumor suppressor 1) is a putative serine/threonine kinase that belongs to the NDR family (1). It is a tumor suppressor that plays a critical role in the maintenance of ploidy. LATS1 localizes to the centrosome and the mitotic spindle and controls G(2)/M transition by negatively regulating cdc2 kinase activity (2,3). It also plays a role in the G1 tetraploidy checkpoint via control of p53 expression (4). 
 LATS1 affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1 (5). LATS1 also binds the phosphorylated form of zyxin, a regulator of actin filament assembly. This interaction promotes localization of zyxin to the mitotic spindle, suggesting a role for actin regulatory proteins during mitosis (6). Decreased expression is associated with breast tumor aggressiveness (7), promoter methylation and loss of heterozygosity. Mutations perturbing LATS1 have been associated with human sarcomas and ovarian sarcomas (8,9). LATS1 knock out mice develop soft-tissue sarcomas, ovarian stromal cell tumors and display a high sensitivity to carcinogenic treatments (10). 
 It has recently been shown that human LATS1 exists in a complex similar to the Drosophila Hippo/Salvador/Lats tumor suppressor network, a complex that regulates proliferation and apoptosis to control growth and shape of the fly. The corresponding human complex contains Hippo and Salvador homologs RASSF1A, WW45 and MST2 and may control mitotic exit (11). LATS1(大的肿瘤抑制基因1)是一个假定的丝氨酸/苏氨酸激酶,属于NDR家族(1)。肿瘤抑制基因在染色体倍性维持中发挥着关键的作用。LATS1集中分布于中心体和有丝分裂纺锤体,它通过负调控cdc2激酶活性来控制G2/M期转换(2,3)。它也可以通过控制p53的表达在G1期四倍体检验点中发挥作用(4)。LATS1通过负调节LIMK1来调控肌动蛋白聚合从而影响细胞质分裂(5)。LATS1也可以结合磷酸化形式的斑联蛋白,一种肌动蛋白丝组装的调节子。这种相互作用可以促进斑联蛋白向有丝分裂的纺锤体转移,表明了其在有丝分裂过程中调节肌动蛋白的作用(6)。LATS1表达下调和乳腺癌侵袭 (7)、启动子甲基化以及杂合性缺失有关。人类肉瘤和卵巢肉瘤也与突变引起的LATS1变化有关(8,9)。LATS1敲除的小鼠能够发生软组织肉瘤、卵巢间质细胞瘤和致癌处理的高敏感性(10)。最近发现人LATS1以复合体的形式存在,该复合体类似于Drosophila Hippo/Salvador/Lats肿瘤抑制基因网络,它通过调节细胞增殖和凋亡来控制果蝇的生长和形状。相应的人类的复合体包括Hipp和Salvador同源蛋白。RASSF1A, WW45和MST2可以控制有丝分裂退出(11)。

  1. Tao, W. et al. (1999) Nat Genet 21, 177-81.
  2. Yang, X. et al. (2001) Oncogene 20, 6516-23.
  3. Xia, H. et al. (2002) Oncogene 21, 1233-41.
  4. Iida, S. et al. (2004) Oncogene 23, 5266-74.
  5. Yang, X. et al. (2004) Nat Cell Biol 6, 609-17.
  6. Hirota, T. et al. (2000) J Cell Biol 149, 1073-86.
  7. Morinaga, N. et al. (2000) Int J Oncol 17, 1125-9.
  8. Hansen, L.L. et al. (2002) Cancer Genet Cytogenet 139, 1-8.
  9. Hisaoka, M. et al. (2002) Lab Invest 82, 1427-35.
  10. St John, M.A. et al. (1999) Nat Genet 21, 182-6.
  11. Guo, C. et al. (2007) Curr Biol 17, 700-5.
  12. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
  13. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.
  14. Zhao, B. et al. (2010) J Cell Sci 123, 4001-6.

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