Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-SEK1/MKK4 (Ser80) Antibody #9155

JNKK   JNKK-1   mkk346   MKK4   mkk47   SAPK/ERK Kinase 1  

No. Size Price
9155S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9155 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 44 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Xenopus,

Specificity / Sensitivity

Phospho-SEK1/MKK4 (Ser80) Antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at serine 80. This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.

Phospho-SEK1/MKK4 (Ser80) Antibody兔多抗能检测内源性Ser80位点磷酸化的SEK1/MKK4蛋白水平。该抗体不与MEK1, MEK2和MKK3蛋白相应磷酸化残基发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser80 of human SEK1/MKK4. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体通过使用与人源SEK1蛋白Ser80位点周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated (lane 1), PDGF-treated (lane 2) or stably transfected with Src (lane 3), using Phospho-SEK1/MKK4 (Ser80) Antibody (upper) or SEK1/MKK4 Antibody #9152 (lower).western blot方法检测细胞提取物:未经处理(1号条带)和PDGF处理的(2号条带)、稳定转染Src(3号条带)的NIH/3T3细胞,使用的抗体是Phospho-SEK1/MKK4 (Ser80) Antibody (上图)和SEK1/MKK4 Antibody #9152 (下图)。

Background

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

SAPK/Erk 激酶(SEK1),也被称为MKK4或Jun激酶的激酶 (JNKK),能激活MAPK同系物SAPK、 JNK,以应答各种细胞应激反应和炎性细胞因子(1-3)。SEK1的活化,是通过MEKK对其Ser257位点和Thr261位点的磷酸化实现的。类似于MEK,SEK也是一种双特异性蛋白激酶,能将SAPK/JNK活化环内的保守位点T*PY* 磷酸化(4)。Akt对SEK1的Ser80位点磷酸化作用,能够抑制SEK1以及应激活化的信号转导(5)。

  1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
  2. Sanchez, I. et al. (1994) Nature 372, 794-798.
  3. Yan, M. et al. (1994) Nature 372, 798-800.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
  5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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